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Journal of Clinical Microbiology, July 1998, p. 2109-2111, Vol. 36, No. 7
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Replication of Bovine Herpesvirus Type 4 in Human Cells In Vitro

László Egyed*

Veterinary Medical Research Institute of the Hungarian Academy of Sciences, Budapest, Hungary

Received 7 November 1997/Returned for modification 4 March 1998/Accepted 7 April 1998

A reference strain (Movár 33/63) of bovine herpesvirus type 4 (BHV-4) was inoculated into 14 different human cell lines and five primary cell cultures representing various human tissues. BHV-4 replicated in two embryonic lung cell lines, MRC-5 and Wistar-38, and in a giant-cell glioblastoma cell culture. Cytopathic effect and intranuclear inclusion bodies were observed in these cells. PCR detected a 10,000-times-higher level of BHV-4 DNA. Titration of the supernatant indicated a 100-fold increase of infectious particles. Since this is the first bovine (human herpesvirus 8 and Epstein-Barr virus related) herpesvirus which replicates on human cells in vitro, the danger of possible human BHV-4 infection should not be ignored.

* Mailing address: Veterinary Medical Research Institute of the Hungarian Academy of Sciences, Budapest, Hungary. Phone: 36-1-252-2455. Fax: 36-1-252-1069. E-mail: Laci{at}novell.vmri.hu.

Journal of Clinical Microbiology, July 1998, p. 2109-2111, Vol. 36, No. 7
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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