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Journal of Clinical Microbiology, July 1998, p. 2117-2119, Vol. 36, No. 7
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Effects of Various Handling and Storage Conditions on Stability of Treponema pallidum DNA in Cerebrospinal Fluid

A. V. Villanueva,1 R. P. Podzorski,2,* and M. P. Reyes1

Division of Infectious Diseases, Department of Internal Medicine,1 and Department of Pathology,2 Wayne State University, Detroit, Michigan

Received 3 November 1997/Returned for modification 5 March 1998/Accepted 10 April 1998

Treponema pallidum DNA from even small numbers of organisms was detectable in cerebrospinal fluid (CSF) stored at room temperature or at 4°C for several hours and in CSF subjected to three freeze-thaw cycles. These results suggest that negative PCR results for T. pallidum from patients diagnosed with T. pallidum invasion of the central nervous system are probably not due to the loss of target DNA prior to testing.

* Corresponding author. Mailing address: Department of Pathology 9374 Scott Hall, Wayne State University School of Medicine, 540 E. Canfield, Detroit, MI 48201-1998. Phone: (313) 745-4609. Fax: (313) 993-8754. E-mail: rpodzor{at}cmb.biosci.wayne.edu.

Journal of Clinical Microbiology, July 1998, p. 2117-2119, Vol. 36, No. 7
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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  • Yamamoto, Y. (2002). PCR in Diagnosis of Infection: Detection of Bacteria in Cerebrospinal Fluids. CVI 9: 508-514 [Full Text]  


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