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Journal of Clinical Microbiology, July 1998, p. 2120-2122, Vol. 36, No. 7
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Comparison of Sample Preparation Methods for Detection of Legionella pneumophila in Culture-Positive Bronchoalveolar Lavage Fluids by PCR

B. Jaulhac,1,* M. Reyrolle,2 Y. K. Sodahlon,1 S. Jarraud,2 M. Kubina,1 H. Monteil,1 Y. Piémont,1 and J. Etienne2

Institut de Bactériologie de la Faculté de Médecine, Université Louis Pasteur et Hopitaux Universitaires de Strasbourg, Strasbourg,1 and Centre National de Référence des Legionella, Laboratoire Central de Microbiologie, Hôpital Edouard Herriot, Lyon,2 France

Received 30 December 1997/Returned for modification 19 February 1998/Accepted 15 April 1998

A prospective study was conducted on 25 Legionella pneumophila culture-positive and 98 culture-negative bronchoalveolar lavage fluid samples to compare two DNA preparation methods: a rapid modified Chelex-based protocol and a proteinase K method. PCR was found to be more sensitive with the Chelex-based method (P = 0.03). No difference was found concerning the inhibition rate.

* Corresponding author. Mailing address: Institut de Bactériologie, 3 rue Koeberlé, 67000 Strasbourg, France. Phone: 33-388-21-19-76. Fax: 33-388-25-11-13. E-mail: jaul-ibs{at}tpgnet.net.

Journal of Clinical Microbiology, July 1998, p. 2120-2122, Vol. 36, No. 7
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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