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Journal of Clinical Microbiology, August 1998, p. 2346-2348, Vol. 36, No. 8
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Enzyme Immunoassay Detecting Teichoic and Lipoteichoic Acids versus Cerebrospinal Fluid Culture and Latex Agglutination for Diagnosis of Streptococcus pneumoniae Meningitis

Kristin Stuertz,1 Imke Merx,1 Helmut Eiffert,2 Erich Schmutzhard,3 Michael Mäder,1 and Roland Nau1,*

Departments of Neurology1 and Medical Microbiology,2 University of Göttingen, Göttingen, Germany, and Department of Neurology, University of Innsbruck, Innsbruck, Austria3

Received 1 October 1997/Returned for modification 9 January 1998/Accepted 5 May 1998

A newly developed enzyme immunoassay (EIA) was used to detect the presence of pneumococcal teichoic and lipoteichoic acids in cerebrospinal fluid (CSF) from patients with Streptococcus pneumoniae meningitis who were being treated with antibiotics. All initial CSF samples, which on culture grew S. pneumoniae, were positive in the EIA. A total of 14 subsequent culture-negative samples gave clear signals in the EIA up to day 15 after the onset of antibiotic treatment. For 11 CSF specimens, culture, microscopy, and latex agglutination were negative while the EIA detected pneumococcal antigens. The EIA did not react either with CSF of patients with meningitis caused by bacteria other than S. pneumoniae or by viral pathogens. In conclusion, this EIA can be a valuable tool for the diagnosis of S. pneumoniae meningitis from CSF samples in cases in which prior antimicrobial therapy minimizes the usefulness of culture or other antigen detection tests.

* Corresponding author. Mailing address: Dept. of Neurology, University of Göttingen, Robert-Koch-Str. 40, D-37075 Göttingen, Germany. Phone: 49-551-398455 or 396684. Fax: 49-551-398405. E-mail: mau{at}gwdg.de.

Journal of Clinical Microbiology, August 1998, p. 2346-2348, Vol. 36, No. 8
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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