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Journal of Clinical Microbiology, August 1998, p. 2363-2365, Vol. 36, No. 8
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Specific Serological Diagnosis of Leprosy with a Recombinant Mycobacterium leprae Protein Purified from a Rapidly Growing Mycobacterial Host

James A. Triccas,1,* Paul W. Roche,1,dagger and Warwick J. Britton1,2

Centenary Institute of Cancer Medicine and Cell Biology, Locked Bag No. 6, Newtown, 2042,1 and Department of Medicine, University of Sydney, Sydney, 2006,2 New South Wales, Australia

Received 20 February 1998/Returned for modification 23 March 1998/Accepted 30 April 1998

In this report we demonstrate the utility of an monoclonal antibody inhibition enzyme-linked immunosorbent assay based on the Mycobacterium leprae 35-kDa protein, purified from the rapidly growing host Mycobacterium smegmatis, for the serodiagnosis of multibacillary leprosy. The assay proved highly specific (97.5%) and sensitive (90%) and compared favorably with two other established methods routinely utilized for leprosy serodiagnosis.

* Corresponding author. Present address: Unité de Génétique Mycobactérienne, Institut Pasteur, 25 rue du Dr. Roux, 75724 Paris Cedex 15, France. Phone: 331-4061-3274. Fax: 331-4568-8843. E-mail: jtriccas{at}pasteur.fr.

dagger Present address: Mycobacterial Research Laboratories, Anandaban Leprosy Hospital, P.O. Box 151, Kathmandu, Nepal.

Journal of Clinical Microbiology, August 1998, p. 2363-2365, Vol. 36, No. 8
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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