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Journal of Clinical Microbiology, August 1998, p. 2383-2385, Vol. 36, No. 8
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Colony Size Can Be Used To Determine the MIC of Fluconazole for Pathogenic Yeasts

Jianping Xu,1,2 Rytas Vilgalys,2 and Thomas G. Mitchell1,*

Department of Microbiology, Duke University Medical Center, Durham, North Carolina 27710,1 and Department of Botany, Duke University, Durham, North Carolina 277082

Received 9 December 1997/Returned for modification 9 March 1998/Accepted 7 May 1998

This report describes a new statistical method for estimating the MIC of fluconazole for yeasts pathogenic to humans. This method is based on comparison of the colony sizes on solid media containing different concentrations of fluconazole. By this method, the MICs of fluconazole for 10 yeast strains were comparable to results obtained by the standard method recommended by the National Committee for Clinical Laboratory Standards. This method is simple to perform and easy to interpret. The turnaround time is faster than other methods. The method should be applicable to the determination MICs of other antifungal drugs for yeasts.

* Corresponding author. Mailing address: Department of Microbiology, Box 3803, Duke University Medical Center, Durham, NC 27710. Phone: (919) 684-5792. Fax: (919) 681-8911. E-mail: mitchell{at}abacus.mc.duke.edu.

Journal of Clinical Microbiology, August 1998, p. 2383-2385, Vol. 36, No. 8
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Xu, J. (2004). Genotype-Environment Interactions of Spontaneous Mutations for Vegetative Fitness in the Human Pathogenic Fungus Cryptococcus neoformans. Genetics 168: 1177-1188 [Abstract] [Full Text]  
  • Xu, J., Onyewu, C., Yoell, H. J., Ali, R. Y., Vilgalys, R. J., Mitchell, T. G. (2001). Dynamic and Heterogeneous Mutations to Fluconazole Resistance in Cryptococcus neoformans. Antimicrob. Agents Chemother. 45: 420-427 [Abstract] [Full Text]  
  • Xu, J., Ramos, A. R., Vilgalys, R., Mitchell, T. G. (2000). Clonal and Spontaneous Origins of Fluconazole Resistance in Candida albicans. J. Clin. Microbiol. 38: 1214-1220 [Abstract] [Full Text]  


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