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Journal of Clinical Microbiology, August 1998, p. 2386-2388, Vol. 36, No. 8
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Comparison of Broth Microdilution Method Using Haemophilus Test Medium and Agar Dilution Method for Susceptibility Testing of Eikenella corrodens

Luis Alcalá,* Fernando García-Garrote, Emilia Cercenado, Teresa Peláez, Gema Ramos, and Emilio Bouza

Servicio de Microbiología y Enfermedades Infecciosas, Hospital General Universitario "Gregorio Marañón," Madrid, Spain

Received 8 December 1997/Returned for modification 26 March 1998/Accepted 29 April 1998

Susceptibility testing of Eikenella corrodens is usually performed by a Mueller-Hinton sheep blood agar dilution (AD) method. However, this method is impractical for testing only a few strains. We compared AD with the broth microdilution method using Haemophilus test medium (HTM) in order to determine the susceptibility of 36 clinical isolates of E. corrodens to eight antimicrobial agents. MICs obtained by the HTM method yielded 95.5 and 84% agreement (within 2 and 1 log2 dilutions, respectively) with those obtained by AD. The HTM method with incubation in CO2 for 48 h was highly reproducible and constitutes an easy alternative for antimicrobial susceptibility testing of E. corrodens.

* Corresponding author. Mailing address: Servicio de Microbiología y Enfermedades Infecciosas, Hospital General Universitario "Gregorio Marañón," Doctor Esquerdo, 46, 28007 Madrid, Spain. Phone: (341) 5868453. Fax: (341) 5044906. E-mail: luisalcala{at}efd.net.

Journal of Clinical Microbiology, August 1998, p. 2386-2388, Vol. 36, No. 8
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Cercenado, E., Cercenado, S., Bouza, E. (2003). In Vitro Activities of Tigecycline (GAR-936) and 12 Other Antimicrobial Agents against 90 Eikenella corrodens Clinical Isolates. Antimicrob. Agents Chemother. 47: 2644-2645 [Abstract] [Full Text]  


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