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Journal of Clinical Microbiology, September 1998, p. 2428-2433, Vol. 36, No. 9
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Rapid Identification of Candida dubliniensis by Indirect Immunofluorescence Based on Differential Localization of Antigens on C. dubliniensis Blastospores and Candida albicans Germ Tubes

Joseba Bikandi,1 Rosario San Millán,1 María D. Moragues,2 Gontzal Cebas,1 Mary Clarke,3 David C. Coleman,3 Derek J. Sullivan,3 Guillermo Quindós,1 and José Pontón1,*

Departamento de Inmunología, Microbiología y Parasitología, Facultad de Medicina y Odontología,1 and Departamento de Enfermería I,2 Universidad del País Vasco, E-48080 Bilbao, Vizcaya, Spain, and Department of Oral Surgery, Oral Medicine and Oral Pathology, School of Dental Science, Trinity College, University of Dublin, Dublin 2, Republic of Ireland3

Received 9 March 1998/Returned for modification 13 May 1998/Accepted 2 June 1998

There is a clear need for the development of a rapid and reliable test for the identification of Candida dubliniensis and for the discrimination of this species from Candida albicans. In the present study we have investigated the potential use of C. dubliniensis-specific antigens as a basis for its identification. We produced an anti-C. dubliniensis serum which, after adsorption with C. albicans blastospores, was found to differentially label C. dubliniensis isolates in an indirect immunofluorescence test. In this test, the antiserum reacted with blastospores and germ tubes of C. dubliniensis and with blastospores of Candida krusei and Rhodotorula rubra but did not react with blastospores of several other Candida species including C. albicans. The antiserum also reacted with C. albicans germ tubes. The anti-C. dubliniensis adsorbed serum reacted with specific components of 25, 28, 37, 40, 52, and 62 kDa in the C. dubliniensis extract and with a variety of antigens from other yeast species. The antigens from non-C. dubliniensis yeasts showing reactivity with the anti-C. dubliniensis adsorbed serum are mostly expressed within the cell walls of these yeast species, and this reactivity does not interfere with the use of the anti-C. dubliniensis adsorbed serum in an indirect immunofluorescence test for the rapid identification of C. dubliniensis.

* Corresponding author. Mailing address: Departamento de Inmunología, Microbiología y Parasitología, Facultad de Medicina y Odontología, Universidad del País Vasco, Apartado 699, E-48080 Bilbao, Vizcaya, Spain. Phone: 4 464 7700, ext. 2746. Fax: 4 464 9266. E-mail: oipposaj{at}lg.ehu.es.

Journal of Clinical Microbiology, September 1998, p. 2428-2433, Vol. 36, No. 9
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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