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Journal of Clinical Microbiology, September 1998, p. 2618-2622, Vol. 36, No. 9
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Phenotypic and Genotypic Characterization of Clinical Strains of CDC Group IVc-2

Gerard J. Osterhout, Joan L. Valentine, and James D. Dick*

Department of Pathology, Division of Microbiology, Johns Hopkins Hospital, Baltimore, Maryland 21287-7093

Received 17 February 1998/Returned for modification 30 March 1998/Accepted 16 June 1998

CDC group IVc-2 is a gram-negative, oxidase-positive, nonfermentative bacillus that has been implicated in human infections, including septicemia and peritonitis. Biochemically it most closely resembles Bordetella bronchiseptica and Alcaligenes sp. Results of cellular fatty acid (CFA) and 16S rRNA gene analysis were combined with biochemical data to assist in identification and classification. The predominant CFAs were hexadecanoic acid (16:0), cis-9-hexadecanoic acid (16:1omega 7c), cis-11-octadecanoic acid (18:1omega 7c), and Delta -cis-9,10-methylenehexadecanoic acid (17:0cyc). Small amounts (2 to 5%) of 3-hydroxytetradecanoic acid (3-OH-14:0), tetradecanoic acid (14:0), 2-hydroxyhexadecanoic acid (2-OH-16:0), and Delta -cis-11,12-methyleneoctadecanoic acid (19:0cyc) were also consistently present. The highest 16S rRNA gene similarity was with Ralstonia eutropha and Ralstonia solanacearum. The CFA and 16S rRNA gene sequence data support the inclusion of CDC group IVc-2 in the recently created genus Ralstonia, which includes R. eutropha, R. pickettii, and R. solanacearum.

* Corresponding author. Mailing address: Department of Pathology, Division of Microbiology, Meyer B1-193, Johns Hopkins Hospital, 600 North Wolfe St., Baltimore, MD 21287-7093. Phone: (410) 955-5077. Fax: (410) 614-8087. E-mail: jdick{at}pathlan.path.jhu.edu.

Journal of Clinical Microbiology, September 1998, p. 2618-2622, Vol. 36, No. 9
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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