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Journal of Clinical Microbiology, September 1998, p. 2629-2633, Vol. 36, No. 9
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Phylogenetic Classification of Trichophyton mentagrophytes Complex Strains Based on DNA Sequences of Nuclear Ribosomal Internal Transcribed Spacer 1 Regions

Koichi Makimura,1,* Takashi Mochizuki,2 Atsuhiko Hasegawa,3 Katsuhisa Uchida,1 Hiuga Saito,1 and Hideyo Yamaguchi1

Teikyo University Institute of Medical Mycology1 and Department of Veterinary Internal Medicine, The University of Tokyo,3 Tokyo, and Department of Dermatology, Kanazawa Medical University, Ishikawa,2 Japan

Received 24 November 1997/Returned for modification 24 January 1998/Accepted 25 June 1998

Using internal transcribed spacer 1 (ITS1) region ribosomal DNA sequences from 37 stock strains and clinical isolates provisionally termed Trichophyton mentagrophytes complex in Japan, we demonstrated the mutual phylogenetic relationships of these strains. Members of this complex were classified into 3 ITS1-homologous groups and 13 ITS1-identical groups by their sequences. ITS1-homologous group I consists of Arthroderma vanbreuseghemii, T. mentagrophytes human isolates, and several strains of T. mentagrophytes animal isolates. Five strains of Arthroderma simii form a cluster comprising ITS1-homologous group II. The Americano-European and African races of Arthroderma benhamiae, T. mentagrophytes var. erinacei, and one strain of a T. mentagrophytes animal isolate constitute ITS1-homologous group III. According to the phylogenetic tree constructed with Trichophyton rubrum as an outgroup, ITS1-homologous groups I and II comprised a monophyletic cluster and ITS1-homologous group III constituted another cluster which was rather distant from the others in the complex. This system was applicable to the phylogenetic analysis of closely related strains. Using this technique, human and animal isolates of T. mentagrophytes were also clearly distinguishable from each other.

* Corresponding author. Mailing address: Teikyo University Institute of Medical Mycology, Otsuka, Hachioji, Tokyo 192-0395, Japan. Phone: 81-426-78-3256. Fax: 81-426-74-9190. E-mail: makimura{at}main.teikyo-u.ac.jp.

Journal of Clinical Microbiology, September 1998, p. 2629-2633, Vol. 36, No. 9
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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