This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Makristathis, A.
Right arrow Articles by Hirschl, A. M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Makristathis, A.
Right arrow Articles by Hirschl, A. M.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, September 1998, p. 2772-2774, Vol. 36, No. 9
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Detection of Helicobacter pylori in Stool Specimens by PCR and Antigen Enzyme Immunoassay

Athanasios Makristathis,1,* Eva Pasching,1 Kurt Schütze,2 Margit Wimmer,1 Manfred L. Rotter,1 and Alexander M. Hirschl1

Department of Clinical Microbiology, Hygiene Institute of the University of Vienna,1 and Medical Department I, Hanusch Hospital,2 Vienna, Austria

Received 21 January 1998/Returned for modification 16 March 1998/Accepted 11 June 1998

A highly sensitive seminested PCR assay to detect Helicobacter pylori DNA in feces was developed. PCR with stool specimens and a novel antigen enzyme immunoassay (EIA) for H. pylori detection in feces were evaluated as diagnostic tools and in follow-up with samples from 63 infected and 37 noninfected persons. Infected individuals received eradication therapy followed by endoscopic follow-up 35 days after the start of treatment. At that time, a second stool specimen was obtained from 55 of these patients. Before eradication, the sensitivity of PCR was 93.7% and that of EIA 88.9%. Specificities were 100 and 94.6%, respectively. Of the 55 follow-up specimens, 41 originated from patients from whom H. pylori had been eradicated. Of these, 21 were still positive by PCR and 13 were positive by EIA, indicating that 1 month may be too short a period for follow-up evaluation of stool specimens by these tests.

* Corresponding author. Mailing address: Department of Clinical Microbiology, University Hospital of Vienna, Währinger Gürtel 18-20, 1090 Vienna, Austria. Phone: 43 (1) 40400-5157. Fax: 43 (1) 40400-5162. E-mail: Athanasios.Makristathis{at}akh-wien.ac.at.

Journal of Clinical Microbiology, September 1998, p. 2772-2774, Vol. 36, No. 9
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Lottspeich, C., Schwarzer, A., Panthel, K., Koletzko, S., Russmann, H. (2007). Evaluation of the Novel Helicobacter pylori ClariRes Real-Time PCR Assay for Detection and Clarithromycin Susceptibility Testing of H. pylori in Stool Specimens from Symptomatic Children. J. Clin. Microbiol. 45: 1718-1722 [Abstract] [Full Text]  
  • Megraud, F., Lehours, P. (2007). Helicobacter pylori Detection and Antimicrobial Susceptibility Testing. Clin. Microbiol. Rev. 20: 280-322 [Abstract] [Full Text]  
  • Schabereiter-Gurtner, C., Hirschl, A. M., Dragosics, B., Hufnagl, P., Puz, S., Kovach, Z., Rotter, M., Makristathis, A. (2004). Novel Real-Time PCR Assay for Detection of Helicobacter pylori Infection and Simultaneous Clarithromycin Susceptibility Testing of Stool and Biopsy Specimens. J. Clin. Microbiol. 42: 4512-4518 [Abstract] [Full Text]  
  • Harper, C. G., Whary, M. T., Feng, Y., Rhinehart, H. L., Wells, R. S., Xu, S., Taylor, N. S., Fox, J. G. (2003). Comparison of Diagnostic Techniques for Helicobacter cetorum Infection in Wild Atlantic Bottlenose Dolphins (Tursiops truncatus). J. Clin. Microbiol. 41: 2842-2848 [Abstract] [Full Text]  
  • Suzuki, N., Wakasugi, M., Nakaya, S., Kokubo, N., Sato, M., Kajiyama, H., Takahashi, R., Hirata, H., Ezure, Y., Fukuda, Y., Shimoyama, T. (2002). Catalase, a Specific Antigen in the Feces of Human Subjects Infected with Helicobacter pylori. CVI 9: 784-788 [Abstract] [Full Text]  
  • Suzuki, N., Wakasugi, M., Nakaya, S., Okada, K., Mochida, R., Sato, M., Kajiyama, H., Takahashi, R., Hirata, H., Ezure, Y., Koga, Y., Fukuda, Y., Shimoyama, T. (2002). Production and Application of New Monoclonal Antibodies Specific for a Fecal Helicobacter pylori Antigen. CVI 9: 75-78 [Abstract] [Full Text]  
  • Goosen, C., Theron, J., Ntsala, M., Maree, F. F., Olckers, A., Botha, S. J., Lastovica, A. J., van der Merwe, S. W. (2002). Evaluation of a Novel Heminested PCR Assay Based on the Phosphoglucosamine Mutase Gene for Detection of Helicobacter pylori in Saliva and Dental Plaque. J. Clin. Microbiol. 40: 205-209 [Abstract] [Full Text]  
  • KABIR, S. (2001). Detection of Helicobacter pylori in faeces by culture, PCR and enzyme immunoassay. J Med Microbiol 50: 1021-1029 [Abstract] [Full Text]  
  • Monteiro, L., Gras, N., Megraud, F. (2001). Magnetic Immuno-PCR Assay with Inhibitor Removal for Direct Detection of Helicobacter pylori in Human Feces. J. Clin. Microbiol. 39: 3778-3780 [Abstract] [Full Text]  
  • Roth, D. E., Taylor, D. N., Gilman, R. H., Meza, R., Katz, U., Bautista, C., Cabrera, L., Velapatino, B., Lebron, C., Razuri, M., Watanabe, J., Monath, T., The Gastrointestinal Physiology Working Group, (2001). Posttreatment Follow-Up of Helicobacter pylori Infection Using a Stool Antigen Immunoassay. CVI 8: 718-723 [Abstract] [Full Text]  
  • Kato, S., Tachikawa, T., Ozawa, K., Konno, M., Okuda, M., Fujisawa, T., Nakazato, Y., Tajiri, H., Iinuma, K. (2001). Urine-Based Enzyme-Linked Immunosorbent Assay for the Detection of Helicobacter pylori Infection in Children. Pediatrics 107 : e87-e87 [Abstract] [Full Text]  
  • VAIRA, D, VAKIL, N (2001). Blood, urine, stool, breath, money, and Helicobacter pylori. Gut 48: 287-289 [Full Text]  
  • Makristathis, A., Barousch, W., Pasching, E., Binder, C., Kuderna, C., Apfalter, P., Rotter, M. L., Hirschl, A. M. (2000). Two Enzyme Immunoassays and PCR for Detection of Helicobacter pylori in Stool Specimens from Pediatric Patients before and after Eradication Therapy. J. Clin. Microbiol. 38: 3710-3714 [Abstract] [Full Text]  
  • Argyros, F. C., Ghosh, M., Huang, L., Masubuchi, N., Cave, D. R., Grübel, P. (2000). Evaluation of a PCR Primer Based on the Isocitrate Dehydrogenase Gene for Detection of Helicobacter pylori in Feces. J. Clin. Microbiol. 38: 3755-3758 [Abstract] [Full Text]  
  • Shepherd, A. J, Williams, C. L, Doherty, C. P, Hossack, M., Preston, T., McColl, K. E L, Weaver, L. T (2000). Comparison of an enzyme immunoassay for the detection of Helicobacter pylori antigens in the faeces with the urea breath test. Arch. Dis. Child. 83: 268-270 [Abstract] [Full Text]  
  • Braden, B., Posselt, H.-G., Ahrens, P., Kitz, R., Dietrich, C. F., Caspary, W. F. (2000). New Immunoassay in Stool Provides an Accurate Noninvasive Diagnostic Method for Helicobacter pylori Screening in Children. Pediatrics 106: 115-117 [Abstract] [Full Text]  
  • Peterson, W. L., Fendrick, A. M., Cave, D. R., Peura, D. A., Garabedian-Ruffalo, S. M., Laine, L. (2000). Helicobacter pylori-Related Disease: Guidelines for Testing and Treatment. Arch Intern Med 160: 1285-1291 [Abstract] [Full Text]  
  • Ballam, L D, Mendall, M A, Asante, M, Morris, J, Strachan, D P, Whincup, P H, Cook, D G (2000). Western blotting is useful in the salivary diagnosis of Helicobacter pylori infection. J. Clin. Pathol. 53: 314-317 [Abstract] [Full Text]  
  • Oderda, G., Rapa, A., Ronchi, B., Lerro, P, Pastore, M., Staiano, A., de'Angelis, G L, Strisciuglio, P (2000). Detection of Helicobacter pylori in stool specimens by non-invasive antigen enzyme immunoassay in children: multicentre Italian study. BMJ 320: 347-348 [Full Text]  
  • Braden, B., Teuber, G., Dietrich, C. F, Caspary, W. F, Lembcke, B. (2000). Comparison of new faecal antigen test with 13C-urea breath test for detecting Helicobacter pylori infection and monitoring eradication treatment: prospective clinical evaluation. BMJ 320: 148-148 [Full Text]  
  • Gramley, W. A., Asghar, A., Frierson, H. F. Jr., Powell, S. M. (1999). Detection of Helicobacter pylori DNA in Fecal Samples from Infected Individuals. J. Clin. Microbiol. 37: 2236-2240 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»