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Journal of Clinical Microbiology, September 1998, p. 2782-2783, Vol. 36, No. 9
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Isolation of Francisella tularensis by Centrifugation of Shell Vial Cell Culture from an Inoculation Eschar

Pierre-Edouard Fournier,1 Lionel Bernabeu,1 Bernard Schubert,2 Maryse Mutillod,1 Veronique Roux,1 and Didier Raoult1,*

Unité des Rickettsies, CNRS:UPRESA 6020, Faculté de Médecine, Université de la Méditerranée, 13385 Marseille Cedex 05,1 and Service de Dermatologie-Vénéréologie, Hopital E. Muller, 68070 Mulhouse Cedex,2 France

Received 20 February 1998/Returned for modification 24 April 1998/Accepted 21 May 1998

A 52-year-old man was admitted to the hospital following the development of an inoculation eschar and fever six days after being bitten by a tick. He was clinically diagnosed as suffering from rickettsiosis. Eschar biopsy cultures on standard bacteriological media remained sterile. However, inoculation of cells with the homogenized specimen by the centrifugation-shell vial technique (M. Marrero and D. Raoult, Am. J. Trop. Med. Hyg. 40:197-199, 1989) resulted in the recovery of a bacterium. Determination of the sequence of the 16S rRNA gene amplified from the organism and comparison of the sequence to other sequences identified it as a strain of Francisella tularensis, whereas the specific serology was still negative. Our findings demonstrate that the centrifugation-cell culture, which is a tool for investigation of tick-transmitted diseases, may have the potential to serve as a method for the cultural isolation of F. tularensis.

* Corresponding author. Mailing address: Unité des rickettsies, CNRS:UPRESA 6020, Faculté de Médecine, Université de la Méditerranée, 27, Boulevard Jean Moulin, 13385 Marseille cedex 05, France. Phone: (33) 04 91 38 55 17. Fax: (33) 04 91 83 03 90. E-mail: DidierRaoult{at}medecine.univ.mrs.fr.

Journal of Clinical Microbiology, September 1998, p. 2782-2783, Vol. 36, No. 9
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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