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Journal of Clinical Microbiology, September 1998, p. 2784-2785, Vol. 36, No. 9
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Antimicrobial Susceptibility of Helicobacter pylori Determined by the E Test Using Tetrazolium Egg Yolk Agar

Yanet Valdez,1 Billie Velapatiño,1 Robert H. Gilman,1,2,dagger ,* Vilma Gutierrez,1 and Carlos León1

Department of Pathology, Universidad Peruana Cayetano Heredia, Lima, Peru,1 and the Department of International Health, Johns Hopkins University, Baltimore, Maryland2

Received 9 December 1997/Returned for modification 26 March 1998/Accepted 22 May 1998

Metronidazole and tetracycline E tests were compared to an agar dilution method for the antimicrobial susceptibility testing of Helicobacter pylori. Sixteen strains were tested by using tetrazolium egg yolk (TEY) agar. The characteristic E test inhibition ellipse was clearer on TEY agar than on standard blood agar and gave results comparable to those of the agar dilution test. The use of TEY medium is preferable to that of blood agar medium in E test MIC determinations for H. pylori.

* Corresponding author. Mailing address: Dept. of International Health, Room 5521, Hopkins School of Hygiene, 615 N. Wolfe St., Baltimore, MD 21205. Phone: (410) 955-6964. Fax: (410) 550-6733. E-mail: Rgilman{at}jhsph.edu.

dagger Member of the Gastrointestinal Physiology Working Group. The other members are Robert Berendson, Raul Leon-Barua, Alberto Ramirez-Ramos, and Sixto Recavarren-Arce, Department of Pathology, Universidad Peruana Cayetano Heredia, Lima, Peru, and Jose Watanabe, Japanese Peruvian Polyclinic, Lima, Peru.

Journal of Clinical Microbiology, September 1998, p. 2784-2785, Vol. 36, No. 9
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

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