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Journal of Clinical Microbiology, January 1999, p. 103-109, Vol. 37, No. 1
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Typing of Listeria monocytogenes Strains by Repetitive Element Sequence-Based PCR

B. Jersek,1,* P. Gilot,2 M. Gubina,3 N. Klun,4 J. Mehle,5 E. Tcherneva,6 N. Rijpens,1 and L. Herman1

Centre of Agricultural Research, Department of Animal Product Quality (DVK), B-9090 Melle,1 and National Reference Center for Listeriosis, Institute of Hygiene and Epidemiology, B-1050 Brussels,2 Belgium; Medical Faculty, Institute of Microbiology3 and Veterinary Faculty,5 University of Ljubljana, and Department of Sanitary Microbiology, Institute of Public Health of the Republic of Slovenia,4 1000 Ljubljana, Slovenia; and Central Veterinary Research Institute, 1606 Sofia, Bulgaria6

Received 28 May 1998/Returned for modification 24 July 1998/Accepted 21 October 1998

Listeria monocytogenes strains possess short repetitive extragenic palindromic (REP) elements and enterobacterial repetitive intergenic consensus (ERIC) sequences. We used repetitive element sequence-based PCR (rep-PCR) to evaluate the potential of REP and ERIC elements for typing L. monocytogenes strains isolated from humans, animals, and foods. On the basis of rep-PCR fingerprints, L. monocytogenes strains were divided into four major clusters matching origin of isolation. rep-PCR fingerprints of human and animal isolates were different from those of food isolates. Computer evaluation of rep-PCR fingerprints allowed discrimination among the tested serotypes 1/2a, 1/2b, 1/2c, 3b, and 4b within each major cluster. The index of discrimination calculated for 52 epidemiologically unrelated isolates of L. monocytogenes was 0.98 for REP- and ERIC-PCR. Our results suggest that rep-PCR can provide an alternative method for L. monocytogenes typing.

* Corresponding author. Present address: Biotechnical Faculty, University of Ljubljana, Jamnikarjeva 101, 1000 Ljubljana, Slovenia. Phone: 386-61-1231161. Fax: 386-61-266296. E-mail: Barbara.Jersek{at}BF.UNI-LJ.SI.

Journal of Clinical Microbiology, January 1999, p. 103-109, Vol. 37, No. 1
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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