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Journal of Clinical Microbiology, January 1999, p. 202-205, Vol. 37, No. 1
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Rapid Identification of Candida glabrata by Using a Dipstick To Detect Trehalase-Generated Glucose

Heidrun Peltroche-Llacsahuanga, Norbert Schnitzler, Rudolf Lütticken, and Gerhard Haase*

Institute of Medical Microbiology, University Hospital RWTH Aachen, D-52057 Aachen, Germany

Received 16 April 1998/Returned for modification 15 June 1998/Accepted 26 September 1998

Candida glabrata is a yeast frequently isolated from human specimens. Based upon its well-known ability to rapidly hydrolyze trehalose, we have developed a novel and cost-effective test incubating one yeast colony emulsified in 50 µl of citrate buffer (0.1 M [pH 5.0]) containing 4% (wt/vol) trehalose for 3 h at 37°C. Trehalase-generated glucose is detected with a commercially available dipstick (range, 1.0 to 50 g/liter). For evaluation, consecutive clinical isolates and several reference strains of C. glabrata (n = 160), C. albicans (n = 120), and other yeast species with potential ability for utilization of trehalose (C. dubliniensis, n = 11; C. famata, n = 15; C. guilliermondii, n = 5; C. lusitaniae, n = 16; C. parapsilosis, n = 20; C. tropicalis, n = 34; C. viswanathii, n = 5; Pichia angusta, n = 2; C. zeylanoides, n = 2; Saccharomyces cerevisiae, n = 16; C. neoformans, n = 7) were tested. Identification of C. glabrata is achieved within 3 h, with a specificity of 99.1% and a sensitivity of 98.8% when grown on Sabouraud dextrose agar supplemented with 4% glucose.

* Corresponding author. Mailing address: Institute of Medical Microbiology, University Hospital RWTH Aachen, D-52057 Aachen, Germany. Phone: 49 241 8089 515. Fax: 49 241 8888 483. E-mail: haase{at}alpha.imib.rwth-aachen.de.

Journal of Clinical Microbiology, January 1999, p. 202-205, Vol. 37, No. 1
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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