This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Steinmetz, I.
Right arrow Articles by White, N. J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Steinmetz, I.
Right arrow Articles by White, N. J.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, January 1999, p. 225-228, Vol. 37, No. 1
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Rapid Identification of Burkholderia pseudomallei by Latex Agglutination Based on an Exopolysaccharide-Specific Monoclonal Antibody

I. Steinmetz,1,* A. Reganzerowski,1 B. Brenneke,1 S. Häussler,1 A. Simpson,2 and N. J. White2

Institute of Medical Microbiology, Hannover Medical School, 30625 Hannover, Germany,1 and Faculty of Tropical Medicine, Mahidol University, Bangkok 10400, Thailand2

Received 8 June 1998/Returned for modification 18 August 1998/Accepted 13 October 1998

We recently identified a constitutively expressed exopolysaccharide of Burkholderia pseudomallei which is composed of a unique linear tetrasaccharide repeating unit consisting of three galactose residues and one 3-deoxy-D-manno-2-octulosonic acid residue. In this study we developed a latex agglutination test based on monoclonal antibody 3015, which is specific for this exopolysaccharide, and evaluated this test for rapid identification of B. pseudomallei grown on agar plates. All 74 environmental and clinical B. pseudomallei strains tested, originating from different areas of Southeast Asia, northern Australia, and Africa, showed a strong and specific agglutination. B. pseudomallei-like organisms and a variety of other bacteria did not react. In conclusion this monoclonal antibody-based test is a simple, rapid, and highly specific method for identifying B. pseudomallei culture isolates from different geographic areas.

* Corresponding author. Mailing address: Medical Microbiology, Hannover Medical School, Carl-Neuberg-Str. 1, 30625 Hannover, Germany. Phone: 0511-532-4352. Fax: 0511-532-4366. E-mail: Steinmetz.Ivo{at}MH-Hannover.DE.

Journal of Clinical Microbiology, January 1999, p. 225-228, Vol. 37, No. 1
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Amornchai, P., Chierakul, W., Wuthiekanun, V., Mahakhunkijcharoen, Y., Phetsouvanh, R., Currie, B. J., Newton, P. N., van Vinh Chau, N., Wongratanacheewin, S., Day, N. P. J., Peacock, S. J. (2007). Accuracy of Burkholderia pseudomallei Identification Using the API 20NE System and a Latex Agglutination Test. J. Clin. Microbiol. 45: 3774-3776 [Abstract] [Full Text]  
  • Supaprom, C., Wang, D., Leelayuwat, C., Thaewpia, W., Susaengrat, W., Koh, V., Ooi, E. E., Lertmemongkolchai, G., Liu, Y. (2007). Development of Real-Time PCR Assays and Evaluation of Their Potential Use for Rapid Detection of Burkholderia pseudomallei in Clinical Blood Specimens. J. Clin. Microbiol. 45: 2894-2901 [Abstract] [Full Text]  
  • Zou, N., Newsome, T., Li, B., Tsai, S., Lo, S.-C. (2007). Human Single-Chain Fv Antibodies Against Burkholderia mallei and Burkholderia pseudomallei. Exp. Biol. Med. 232: 550-556 [Abstract] [Full Text]  
  • Cheng, A. C., Currie, B. J. (2005). Melioidosis: Epidemiology, Pathophysiology, and Management. Clin. Microbiol. Rev. 18: 383-416 [Abstract] [Full Text]  
  • Currie, B.J. (2003). Melioidosis: an important cause of pneumonia in residents of and travellers returned from endemic regions. Eur Respir J 22: 542-550 [Abstract] [Full Text]  
  • Ramisse, V., Balandreau, J., Thibault, F., Vidal, D., Vergnaud, G., Normand, P. (2003). DNA-DNA hybridization study of Burkholderia species using genomic DNA macro-array analysis coupled to reverse genome probing. Int. J. Syst. Evol. Microbiol. 53: 739-746 [Abstract] [Full Text]  
  • ATKINS, T., PRIOR, R., MACK, K., RUSSELL, P., NELSON, M., PRIOR, J., ELLIS, J., OYSTON, P. C. F., DOUGAN, G., TITBALL, R. W. (2002). Characterisation of an acapsular mutant of Burkholderia pseudomallei identified by signature tagged mutagenesis. J Med Microbiol 51: 539-553 [Abstract] [Full Text]  
  • Schülin, T., Steinmetz, I. (2001). Chronic Melioidosis in a Patient with Cystic Fibrosis. J. Clin. Microbiol. 39: 1676-1677 [Abstract] [Full Text]  
  • Reckseidler, S. L., DeShazer, D., Sokol, P. A., Woods, D. E. (2001). Detection of Bacterial Virulence Genes by Subtractive Hybridization: Identification of Capsular Polysaccharide of Burkholderia pseudomallei as a Major Virulence Determinant. Infect. Immun. 69: 34-44 [Abstract] [Full Text]  
  • ANUNTAGOOL, N., NAIGOWIT, P., PETKANCHANAPONG, V., ARAMSRI, P., PANICHAKUL, T., SIRISINHA, S. (2000). Monoclonal antibody-based rapid identification of Burkholderia pseudomallei in blood culture fluid from patients with community-acquired septicaemia. J Med Microbiol 49: 1075-1078 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»