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Journal of Clinical Microbiology, January 1999, p. 245-247, Vol. 37, No. 1
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.
DNA Fingerprinting of Single Colonies of
Helicobacter pylori from Gastric Cancer Patients Suggests
Infection with a Single Predominant Strain
Stephan
Miehlke,1
Rachel
Thomas,2
Oscar
Guiterrez,3
David Y.
Graham,2 and
Mae F.
Go2,*
Medical Department I, Technical University
Hospital, Dresden, Germany1;
Department
of Medicine, Veterans Affairs Medical Center, Baylor College of
Medicine, Houston, Texas2; and
University Hospital San Juan de Dios, Bogota,
Colombia3
Received 3 June 1998/Returned for modification 18 August
1998/Accepted 13 October 1998
In each of six gastric cancer patients, repetitive extragenic
palindromic PCR DNA fingerprints of 18 single colonies of
Helicobacter pylori from the gastric antrum, corpus, and
cardia were identical and matched that of the parental isolate. In
three additional gastric cancer patients, 17 of 18 single-colony DNA
fingerprints were identical to each other and to the DNA fingerprint of
the corresponding parental isolate.
*
Corresponding author. Mailing address: Veterans Affairs
Medical Center (111 D), 2002 Holcombe Blvd., Houston, TX 77030. Phone: (713) 794-7231. Fax: (713) 790-1040.
Journal of Clinical Microbiology, January 1999, p. 245-247, Vol. 37, No. 1
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.
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