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Journal of Clinical Microbiology, January 1999, p. 245-247, Vol. 37, No. 1
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

DNA Fingerprinting of Single Colonies of Helicobacter pylori from Gastric Cancer Patients Suggests Infection with a Single Predominant Strain

Stephan Miehlke,1 Rachel Thomas,2 Oscar Guiterrez,3 David Y. Graham,2 and Mae F. Go2,*

Medical Department I, Technical University Hospital, Dresden, Germany1; Department of Medicine, Veterans Affairs Medical Center, Baylor College of Medicine, Houston, Texas2; and University Hospital San Juan de Dios, Bogota, Colombia3

Received 3 June 1998/Returned for modification 18 August 1998/Accepted 13 October 1998

In each of six gastric cancer patients, repetitive extragenic palindromic PCR DNA fingerprints of 18 single colonies of Helicobacter pylori from the gastric antrum, corpus, and cardia were identical and matched that of the parental isolate. In three additional gastric cancer patients, 17 of 18 single-colony DNA fingerprints were identical to each other and to the DNA fingerprint of the corresponding parental isolate.


* Corresponding author. Mailing address: Veterans Affairs Medical Center (111 D), 2002 Holcombe Blvd., Houston, TX 77030. Phone: (713) 794-7231. Fax: (713) 790-1040.


Journal of Clinical Microbiology, January 1999, p. 245-247, Vol. 37, No. 1
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.



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