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Journal of Clinical Microbiology, January 1999, p. 35-38, Vol. 37, No. 1
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Development of a Plasmodium PCR for Monitoring Efficacy of Antimalarial Treatment

Liliane Ciceron,1,* Ginette Jaureguiberry,2 Frederick Gay,1 and Martin Danis1

Service de Parasitologie et Unité INSERM 313, Centre Hospitalier-Universitaire Pitié-Salpêtrière, 75013 Paris,1 and EP 1790 CNRS, Laboratoire de Biologie Parasitaire, MNHN, 75005 Paris,2 France

Received 11 May 1998/Returned for modification 22 July 1998/Accepted 14 October 1998

We report in this work a highly sensitive and nonradioactive PCR method for the detection of the four species of parasite causing human malaria. Plasmodium-specific primers corresponding to the small-subunit rRNA genes of the malaria parasite were used, and a 291-bp fragment was amplified. Our results showed a high specificity for the four human Plasmodium species, and we were able to detect one parasite in 50 µl of whole blood. The responses of 12 patients infected with Plasmodium falciparum to antimalarial therapy were monitored by PCR diagnosis and examination of thick blood film for at least 20 min by an experienced microscopist. For one patient this study allowed early diagnosis of therapeutic failure, confirmed 7 days later by examination of the thick blood film. A total of 134 samples were examined; 94 were positive by PCR, and among these 68 were positive by thick blood film examination. The sensitivity of the thick blood film was 72.3% compared to PCR and 60.7% compared to dot blot hybridization.

* Corresponding author. Mailing address: Unité INSERM 313, CHU Pitié-Salpêtrière, 47 Bld. de l'Hôpital, 75013 Paris, France. Phone: 33-1-42-16-01-47. Fax: 33-1-42-16-01-65. E-mail: martin.danis{at}psl.ap-hop-paris.fr.

Journal of Clinical Microbiology, January 1999, p. 35-38, Vol. 37, No. 1
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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