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Journal of Clinical Microbiology, October 1999, p. 3108-3112, Vol. 37, No. 10
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Application of Infrequent-Restriction-Site PCR to Clinical Isolates of Acinetobacter baumannii and Serratia marcescens

Jin-Hong Yoo,1,* Jung-Hyun Choi,1 Wan-Shik Shin,1 Dong-Ho Huh,2 Yoon-Kyung Cho,2 Kyung-Mi Kim,3 Mi-Young Kim,1 and Moon-Won Kang1

Department of Internal Medicine,1 Clinical Research Institute,2 and Hospital Epidemiology & Infection Control Unit,3 College of Medicine, The Catholic University of Korea, Seoul, Korea

Received 15 March 1999/Returned for modification 22 May 1999/Accepted 27 June 1999

We applied infrequent-restriction-site PCR (IRS-PCR) to the investigation of an outbreak caused by 23 isolates of Acinetobacter baumannii in an intensive care unit from November 1996 to May 1997 and a pseudoepidemic caused by 16 isolates of Serratia marcescens in a delivery room from May to September 1996. In the epidemiologic investigation of the outbreak caused by A. baumannii, environmental sampling and screening of all health care workers revealed the same species from the Y piece of a mechanical ventilator and the hands of two health care personnel. IRS-PCR showed that all outbreak-related strains were genotypically identical and that three strains from surveillance cultures were also identical to the outbreak-related strains. In a pseudoepidemic caused by S. marcescens, IRS-PCR identified two different genotypes, and among them one genotype was predominant (15 of 16 [93.8%] isolates). Extensive surveillance failed to find any source of S. marcescens. Validation of the result of IRS-PCR by comparison with that of field inversion gel electrophoresis (FIGE) showed that they were completely concordant. These results suggest that IRS-PCR is comparable to FIGE for molecular epidemiologic studies. In addition, IRS-PCR was less laborious and less time-consuming than FIGE. To our knowledge, this is the first report of the application of IRS-PCR to A. baumannii and S. marcescens.

* Corresponding author. Mailing address: Department of Internal Medicine, St. Mary's Hospital, 62 Yoido-dong, Youngdungpo-Gu, Seoul 150-010, South Korea. Phone: 82-2-3779-1151. Fax: 82-32-340-2669. E-mail: mogul{at}chollian.net.

Journal of Clinical Microbiology, October 1999, p. 3108-3112, Vol. 37, No. 10
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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