This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Smith, H. E.
Right arrow Articles by Smits, M. A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Smith, H. E.
Right arrow Articles by Smits, M. A.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, October 1999, p. 3146-3152, Vol. 37, No. 10
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

The cps Genes of Streptococcus suis Serotypes 1, 2, and 9: Development of Rapid Serotype-Specific PCR Assays

Hilde E. Smith,* Vincent Veenbergen, Joeke van der Velde, Marloes Damman, Henk J. Wisselink, and Mari A. Smits

Department of Bacteriology, DLO-Institute for Animal Science and Health, 8200 AB Lelystad, The Netherlands

Received 28 December 1998/Returned for modification 12 April 1999/Accepted 14 June 1999

We developed three type-specific PCR assays for the rapid and sensitive detection of Streptococcus suis serotype 1 (plus 14), serotype 2 (plus 1/2), and serotype 9 strains in tonsillar specimens from pigs. The PCR primers were based on the sequences of type-specific capsular genes of S. suis serotype 1, 2, and 9 strains. We recently characterized a major part of the capsular biosynthesis (cps) locus of S. suis serotype 2. Here we extended these studies and characterized major parts of the cps loci of S. suis serotypes 1 and 9. Type-specific genes were identified by cross-hybridization experiments between the individual cps genes and chromosomal DNAs from the 35 different serotypes. Four genes of S. suis serotype 1 specifically hybridized with serotype 1 and 14 strains only. Five genes of S. suis serotype 2 specifically hybridized with serotype 2 and 1/2 strains only, and two genes of S. suis serotype 9 specifically hybridized with serotype 9 strains. Until now rapid and sensitive diagnostic tests were available only for pathogenic strains of serotype 2 and highly pathogenic strains of serotype 1. The serotype-specific PCR assays can therefore be useful tools for the identification of serotype 1, 14, 2, 1/2, and 9 strains both for diagnostic purposes and in epidemiological and transmission studies. Therefore, these tests may facilitate control and eradication programs.

* Corresponding author. Mailing address: Department of Bacteriology, DLO-Institute for Animal Science and Health, P.O. Box 65, 8200 AB Lelystad, The Netherlands. Phone: 31.320.238270. Fax: 31.320.238153. E-mail: h.e.smith{at}id.dlo.nl.

Journal of Clinical Microbiology, October 1999, p. 3146-3152, Vol. 37, No. 10
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Kerdsin, A., Oishi, K., Sripakdee, S., Boonkerd, N., Polwichai, P., Nakamura, S., Uchida, R., Sawanpanyalert, P., Dejsirilert, S. (2009). Clonal dissemination of human isolates of Streptococcus suis serotype 14 in Thailand. J Med Microbiol 58: 1508-1513 [Abstract] [Full Text]  
  • Mavroidi, A., Aanensen, D. M., Godoy, D., Skovsted, I. C., Kaltoft, M. S., Reeves, P. R., Bentley, S. D., Spratt, B. G. (2007). Genetic Relatedness of the Streptococcus pneumoniae Capsular Biosynthetic Loci. J. Bacteriol. 189: 7841-7855 [Abstract] [Full Text]  
  • Yang, J., Jin, M., Chen, J., Yang, Y., Zheng, P., Zhang, A., Song, Y., Zhou, H., Chen, H. (2007). Development and evaluation of an immunochromatographic strip for detection of Streptococcus Suis type 2 antibody. jvdi 19: 355-361 [Abstract] [Full Text]  
  • Heidt, M. C., Mohamed, W., Hain, T., Vogt, P. R., Chakraborty, T., Domann, E. (2005). Human Infective Endocarditis Caused by Streptococcus suis Serotype 2. J. Clin. Microbiol. 43: 4898-4901 [Abstract] [Full Text]  
  • Marois, C., Bougeard, S., Gottschalk, M., Kobisch, M. (2004). Multiplex PCR Assay for Detection of Streptococcus suis Species and Serotypes 2 and 1/2 in Tonsils of Live and Dead Pigs. J. Clin. Microbiol. 42: 3169-3175 [Abstract] [Full Text]  
  • de Greeff, A., Hamilton, A., Sutcliffe, I. C., Buys, H., van Alphen, L., Smith, H. E. (2003). Lipoprotein signal peptidase of Streptococcus suis serotype 2. Microbiology 149: 1399-1407 [Abstract] [Full Text]  
  • King, S. J., Leigh, J. A., Heath, P. J., Luque, I., Tarradas, C., Dowson, C. G., Whatmore, A. M. (2002). Development of a Multilocus Sequence Typing Scheme for the Pig Pathogen Streptococcus suis: Identification of Virulent Clones and Potential Capsular Serotype Exchange. J. Clin. Microbiol. 40: 3671-3680 [Abstract] [Full Text]  
  • de Greeff, A., Buys, H., Verhaar, R., van Alphen, L., Smith, H. E. (2002). Distribution of Environmentally Regulated Genes of Streptococcus suis Serotype 2 among S. suis Serotypes and Other Organisms. J. Clin. Microbiol. 40: 3261-3268 [Abstract] [Full Text]  
  • Wisselink, H. J., Joosten, J. J., Smith, H. E. (2002). Multiplex PCR Assays for Simultaneous Detection of Six Major Serotypes and Two Virulence-Associated Phenotypes of Streptococcus suis in Tonsillar Specimens from Pigs. J. Clin. Microbiol. 40: 2922-2929 [Abstract] [Full Text]  
  • Brousseau, R., Hill, J. E., Prefontaine, G., Goh, S.-H., Harel, J., Hemmingsen, S. M. (2001). Streptococcus suis Serotypes Characterized by Analysis of Chaperonin 60 Gene Sequences. Appl. Environ. Microbiol. 67: 4828-4833 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»