This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Costello, E.
Right arrow Articles by Griffin, J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Costello, E.
Right arrow Articles by Griffin, J.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, October 1999, p. 3217-3222, Vol. 37, No. 10
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Study of Restriction Fragment Length Polymorphism Analysis and Spoligotyping for Epidemiological Investigation of Mycobacterium bovis Infection

Eamon Costello,1,* Donnacha O'Grady,1 Orla Flynn,1 Rory O'Brien,2 Mark Rogers,2 Frances Quigley,1 John Egan,1 and John Griffin3

Central Veterinary Research Laboratory, Abbotstown, Castleknock, Dublin 15,1 and National Agricultural and Veterinary Biotechnology Centre2 and Veterinary Epidemiology and Tuberculosis Investigation Unit,3 University College Dublin, Dublin 4, Ireland

Received 7 December 1998/Returned for modification 2 April 1999/Accepted 7 April 1999

Restriction fragment length polymorphism (RFLP) analysis with probes derived from the insertion element IS6110, the direct repeat sequence, and the polymorphic GC-rich sequence (PGRS) and a PCR-based typing method called spacer oligonucleotide typing (spoligotyping) were used to strain type Mycobacterium bovis isolates from the Republic of Ireland. Results were assessed for 452 isolates which were obtained from 233 cattle, 173 badgers, 33 deer, 7 pigs, 5 sheep, and 1 goat. Eighty-five strains were identified by RFLP analysis, and 20 strains were identified by spoligotyping. Twenty percent of the isolates were the most prevalent RFLP type, while 52% of the isolates were the most prevalent spoligotype. Both the prevalent RFLP type and the prevalent spoligotype were identified in isolates from all animal species tested and had a wide geographic distribution. Isolates of some RFLP types and some spoligotypes were clustered in regions consisting of groups of adjoining counties. The PGRS probe gave better differentiation of strains than the IS6110 or DR probes. The majority of isolates from all species carried a single IS6110 copy. In four RFLP types IS6110 polymorphism was associated with deletion of fragments equivalent in size to one or two direct variable repeat sequences. The same range and geographic distribution of strains were found for the majority of isolates from cattle, badgers, and deer. This suggests that transmission of infection between these species is a factor in the epidemiology of M. bovis infection in Ireland.

* Corresponding author. Mailing address: Eamon Costello, Central Veterinary Research Laboratory, Abbotstown, Castleknock, Dublin 15, Ireland. Phone: (353) 1 6072789. Fax: (353) 1 8213010. E-mail: eamoncos{at}indigo.ie.

Journal of Clinical Microbiology, October 1999, p. 3217-3222, Vol. 37, No. 10
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Lari, N., Rindi, L., Bonanni, D., Tortoli, E., Garzelli, C. (2006). Molecular Analysis of Clinical Isolates of Mycobacterium bovis Recovered from Humans in Italy. J. Clin. Microbiol. 44: 4218-4221 [Abstract] [Full Text]  
  • Prodinger, W. M., Brandstatter, A., Naumann, L., Pacciarini, M., Kubica, T., Boschiroli, M. L., Aranaz, A., Nagy, G., Cvetnic, Z., Ocepek, M., Skrypnyk, A., Erler, W., Niemann, S., Pavlik, I., Moser, I. (2005). Characterization of Mycobacterium caprae Isolates from Europe by Mycobacterial Interspersed Repetitive Unit Genotyping. J. Clin. Microbiol. 43: 4984-4992 [Abstract] [Full Text]  
  • Aranaz, A., de Juan, L., Montero, N., Sanchez, C., Galka, M., Delso, C., Alvarez, J., Romero, B., Bezos, J., Vela, A. I., Briones, V., Mateos, A., Dominguez, L. (2004). Bovine Tuberculosis (Mycobacterium bovis) in Wildlife in Spain. J. Clin. Microbiol. 42: 2602-2608 [Abstract] [Full Text]  
  • Kubica, T., Rusch-Gerdes, S., Niemann, S. (2003). Mycobacterium bovis subsp. caprae Caused One-Third of Human M. bovis-Associated Tuberculosis Cases Reported in Germany between 1999 and 2001. J. Clin. Microbiol. 41: 3070-3077 [Abstract] [Full Text]  
  • Mistry, N. F., Iyer, A. M., D'souza, D. T. B., Taylor, G. M., Young, D. B., Antia, N. H. (2002). Spoligotyping of Mycobacterium tuberculosis Isolates from Multiple-Drug-Resistant Tuberculosis Patients from Bombay, India. J. Clin. Microbiol. 40: 2677-2680 [Abstract] [Full Text]  
  • Prodinger, W. M., Eigentler, A., Allerberger, F., Schonbauer, M., Glawischnig, W. (2002). Infection of Red Deer, Cattle, and Humans with Mycobacterium bovis subsp. caprae in Western Austria. J. Clin. Microbiol. 40: 2270-2272 [Abstract] [Full Text]  
  • Skuce, R. A., McCorry, T. P., McCarroll, J. F., Roring, S. M. M., Scott, A. N., Brittain, D., Hughes, S. L., Hewinson, R. G., Neill, S. D. (2002). Discrimination of Mycobacterium tuberculosis complex bacteria using novel VNTR-PCR targets. Microbiology 148: 519-528 [Abstract] [Full Text]  
  • Cameron, H., O'Brien, R., Murray, A., Cryan, B., Hone, R., Rogers, M. (2001). Evaluation of the Mycobacterium bovis Restriction Fragment Length Polymorphism Probe pUCD, in Combination with the Direct Repeat Probe, for Molecular Typing of Mycobacterium tuberculosis Strains in Ireland. J. Clin. Microbiol. 39: 4404-4406 [Abstract] [Full Text]  
  • Haddad, N., Ostyn, A., Karoui, C., Masselot, M., Thorel, M. F., Hughes, S. L., Inwald, J., Hewinson, R. G., Durand, B. (2001). Spoligotype Diversity of Mycobacterium bovis Strains Isolated in France from 1979 to 2000. J. Clin. Microbiol. 39: 3623-3632 [Abstract] [Full Text]  
  • O'Brien, R., Danilowicz, B. S., Bailey, L., Flynn, O., Costello, E., O'Grady, D., Rogers, M. (2000). Characterization of the Mycobacterium bovis Restriction Fragment Length Polymorphism DNA Probe pUCD and Performance Comparison with Standard Methods. J. Clin. Microbiol. 38: 3362-3369 [Abstract] [Full Text]  
  • O'Brien, R., Flynn, O., Costello, E., O'Grady, D., Rogers, M. (2000). Identification of a Novel DNA Probe for Strain Typing Mycobacterium bovis by Restriction Fragment Length Polymorphism Analysis. J. Clin. Microbiol. 38: 1723-1730 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»