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Journal of Clinical Microbiology, October 1999, p. 3357-3361, Vol. 37, No. 10
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Molecular Characterization of a Shiga Toxigenic Escherichia coli O113:H21 Strain Lacking eae Responsible for a Cluster of Cases of Hemolytic-Uremic Syndrome

Adrienne W. Paton,1 Matthew C. Woodrow,1 Robyn M. Doyle,2 Janice A. Lanser,2 and James C. Paton1,*

Molecular Microbiology Unit, Women's and Children's Hospital, North Adelaide, S.A. 5006,1 and Division of Clinical Microbiology, Institute of Medical and Veterinary Science, Adelaide, S.A. 5000,2 Australia

Received 17 May 1999/Returned for modification 3 July 1999/Accepted 14 July 1999

Shiga toxigenic Escherichia coli (STEC) strains are a diverse group of organisms capable of causing severe gastrointestinal disease in humans. Within the STEC family, certain strains appear to have greater virulence for humans. STEC strains carrying eae and belonging to serogroup O157 or O111 have been responsible for the vast majority of outbreaks of STEC disease reported to date. Here we describe a STEC O113:H21 strain lacking eae that was responsible for a cluster of three cases of hemolytic-uremic syndrome. This strain produces a single Stx2-related toxin and adheres efficiently to Henle 407 cells.

* Corresponding author. Mailing address: Molecular Microbiology Unit, Women's and Children's Hospital, North Adelaide, S.A. 5006, Australia. Phone: 61-8-8204 6302. Fax: 61-8-8204 6051. E-mail: patonj{at}wch.sa.gov.au.

Journal of Clinical Microbiology, October 1999, p. 3357-3361, Vol. 37, No. 10
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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