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Journal of Clinical Microbiology, October 1999, p. 3399-3401, Vol. 37, No. 10
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Optimizing Culture of Chlamydia pneumoniae by Using Multiple Centrifugations

Janet M. Pruckler,1,* Nirupama Masse,1 Valerie A. Stevens,1 Liu Gang,2 Yonghong Yang,2 Elizabeth R. Zell,1 Scott F. Dowell,1 and Barry S. Fields1

Division of Bacterial and Mycotic Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia 30333,1 and Beijing Children's Hospital, Beijing, China2

Received 8 March 1999/Returned for modification 28 April 1999/Accepted 25 June 1999

Three methods for the recovery of Chlamydia pneumoniae from spiked nasopharyngeal and blood specimens, including extended culture and additional centrifugations, were compared. Additional centrifugations and a 7-day culture time resulted in a 500- to 5,000-fold increase in the number of detectable inclusion-forming units.

* Corresponding author. Mailing address: Centers for Disease Control and Prevention, 1600 Clifton Rd., N.E., Mailstop C02, Atlanta GA 30333. Phone: (404) 639-3563. Fax: (404) 639-4215. E-mail: jmp5{at}cdc.gov.

Journal of Clinical Microbiology, October 1999, p. 3399-3401, Vol. 37, No. 10
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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