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Journal of Clinical Microbiology, November 1999, p. 3491-3496, Vol. 37, No. 11
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Escherichia coli O157:H7 and O157:Hminus Strains That Do Not Produce Shiga Toxin: Phenotypic and Genetic Characterization of Isolates Associated with Diarrhea and Hemolytic-Uremic Syndrome

H. Schmidt,1 J. Scheef,1 H. I. Huppertz,2 M. Frosch,1 and H. Karch1,*

Institut für Hygiene und Mikrobiologie der Universität Würzburg1 and Kinderklinik der Universität Würzburg,2 97080 Würzburg, Germany

Received 28 April 1999/Returned for modification 17 July 1999/Accepted 23 July 1999

We have isolated one sorbitol-nonfermenting (SNF) Escherichia coli O157:H7 isolate and five sorbitol-fermenting (SF) E. coli O157:H- isolates that do not contain Shiga toxin (Stx) genes (stx). Isolates originated from patients with diarrhea (n = 4) and hemolytic-uremic syndrome (HUS) (n = 2). All isolates harbored a chromosomal eae gene encoding gamma-intimin as well as the plasmid genes E-hly and etp. The E. coli O157:H7 isolate was katP and espP positive. Respective sera obtained from the patient with HUS contained antibodies to the O157 lipopolysaccharide antigen. The stx-negative E. coli O157:H7 isolate is genetically related to stx-positive SNF E. coli O157:H7. All stx-negative SF E. coli O157:H- isolates belong to the same genetic cluster and are closely related to stx-positive SF E. coli O157:H- isolates. Our data indicate that stx-negative E. coli O157:H7/H- variants may occur at a low frequency and cannot be recognized by diagnostic methods that target Stx.

* Corresponding author. Mailing address: Institut für Hygiene und Mikrobiologie der Universität Würzburg, Bau 17, Josef-Schneider-Str. 2, D-97080 Würzburg. Phone: 49/931/201/5162. Fax: 49/931/201-5166. E-mail: hkarch{at}hygiene.uni-wuerzburg.de.

Journal of Clinical Microbiology, November 1999, p. 3491-3496, Vol. 37, No. 11
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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