This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Pincus, D. H.
Right arrow Articles by Hearn, V.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Pincus, D. H.
Right arrow Articles by Hearn, V.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, November 1999, p. 3533-3539, Vol. 37, No. 11
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Rapid Identification of Candida dubliniensis with Commercial Yeast Identification Systems

D. H. Pincus,1,* D. C. Coleman,2 W. R. Pruitt,3 A. A. Padhye,3 I. F. Salkin,4 M. Geimer,1 A. Bassel,1 D. J. Sullivan,2 M. Clarke,2 and V. Hearn2

bioMérieux, Inc., Hazelwood, Missouri1; Department of Oral Medicine and Oral Pathology, School of Dental Science and Dublin Dental Hospital, Trinity College, University of Dublin, Dublin 2, Republic of Ireland2; Mycotic Diseases Branch, Division of Bacterial and Mycotic Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia3; and Wadsworth Center for Laboratories and Research, New York State Department of Health, Albany, New York4

Received 16 April 1999/Returned for modification 12 July 1999/Accepted 2 August 1999

Candida dubliniensis is a newly described species that is closely related phylogenetically to Candida albicans and that is commonly associated with oral candidiasis in human immunodeficiency virus-positive patients. Several recent studies have attempted to elucidate phenotypic and genotypic characteristics of use in separating the two species. However, results obtained with simple phenotypic tests were too variable and tests that provided more definitive data were too complex for routine use in the clinical laboratory setting. The objective of this study was to determine if reproducible identification of C. dubliniensis could be obtained with commercial identification kits. The substrate reactivity profiles of 80 C. dubliniensis isolates were obtained by using the API 20C AUX, ID 32 C, RapID Yeast Plus, VITEK YBC, and VITEK 2 ID-YST systems. The percentages of C. dubliniensis isolates capable of assimilating or hydrolyzing each substrate were compared with the percentages from the C. albicans profiles in each kit's database, and the results were expressed as percent C. dubliniensis and percent C. albicans. Any substrate that showed >50% difference in reactivity was considered useful in differentiating the species. In addition, assimilation of methyl-alpha -D-glucoside (MDG), D-trehalose (TRE), and D-xylose (XYL) by the same isolates was investigated by the traditional procedure of Wickerham and Burton (L. J. Wickerham and K. A. Burton, J. Bacteriol. 56:363-371, 1948). At 48 h (the time recommended by the manufacturer for its new database), we found that the assimilation of four carbohydrates in the API 20C AUX system could be used to distinguish the species, i.e., glycerol (GLY; 88 and 14%), XYL (0 and 88%), MDG (0 and 85%), and TRE (15 and 97%). Similarly, results with the ID 32 C system at 48 h showed that XYL (0 and 98%), MDG (0 and 98%), lactate (LAT; 0 and 96%), and TRE (30 and 96%) could be used to separate the two species. Phosphatase (PHS; 9 and 76%) and alpha -D-glucosidase (23 and 94%) proved to be the most useful for separation of the species in the RapID Yeast Plus system. While at 24 h the profiles obtained with the VITEK YBC system showed that MDG (10 and 95%), XYL (0 and 95%), and GLY (26 and 80%) could be used to separate the two species, at 48 h only XYL (6 and 95%) could be used to separate the two species. The most useful substrates in the VITEK 2 ID-YST system were TRE (1 and 89%), MDG (1 and 99%), LAT (4 and 98%), and PHS (83 and 1%). While the latter kit was not yet commercially available at the time of the study, it would appear to be the most valuable for the identification of C. dubliniensis. Although assimilation of MDG, TRE, and XYL proved to be the most useful for species differentiation by the majority of commercial systems, the results with these carbohydrates by the Wickerham and Burton procedure were essentially the same for both species, albeit following protracted incubation. Thus, it is the rapidity of the assimilation achieved with the commercial systems that allows the differentiation of C. dubliniensis from C. albicans.

* Corresponding author. Mailing address: Dept. 015-B1/L3, bioMérieux, Inc., 595 Anglum Rd., Hazelwood, MO 63042-2320. Phone: (314) 731-7456. Fax: (314) 731-7454. E-mail: dave_pincus{at}na.biomerieux.com.

Journal of Clinical Microbiology, November 1999, p. 3533-3539, Vol. 37, No. 11
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • McManus, B. A., Coleman, D. C., Moran, G., Pinjon, E., Diogo, D., Bougnoux, M.-E., Borecka-Melkusova, S., Bujdakova, H., Murphy, P., d'Enfert, C., Sullivan, D. J. (2008). Multilocus Sequence Typing Reveals that the Population Structure of Candida dubliniensis Is Significantly Less Divergent than That of Candida albicans. J. Clin. Microbiol. 46: 652-664 [Abstract] [Full Text]  
  • Hata, D. J., Hall, L., Fothergill, A. W., Larone, D. H., Wengenack, N. L. (2007). Multicenter Evaluation of the New VITEK 2 Advanced Colorimetric Yeast Identification Card. J. Clin. Microbiol. 45: 1087-1092 [Abstract] [Full Text]  
  • Romeo, O., Racco, C., Criseo, G. (2006). Amplification of the Hyphal Wall Protein 1 Gene To Distinguish Candida albicans from Candida dubliniensis.. J. Clin. Microbiol. 44: 2590-2592 [Abstract] [Full Text]  
  • Sahand, I. H., Moragues, M. D., Eraso, E., Villar-Vidal, M., Quindos, G., Ponton, J. (2005). Supplementation of CHROMagar Candida Medium with Pal's Medium for Rapid Identification of Candida dubliniensis. J. Clin. Microbiol. 43: 5768-5770 [Abstract] [Full Text]  
  • Al Mosaid, A., Sullivan, D. J., Polacheck, I., Shaheen, F. A., Soliman, O., Al Hedaithy, S., Al Thawad, S., Kabadaya, M., Coleman, D. C. (2005). Novel 5-Flucytosine-Resistant Clade of Candida dubliniensis from Saudi Arabia and Egypt Identified by Cd25 Fingerprinting. J. Clin. Microbiol. 43: 4026-4036 [Abstract] [Full Text]  
  • Carr, M. J., Clarke, S., O'Connell, F., Sullivan, D. J., Coleman, D. C., O'Connell, B. (2005). First Reported Case of Endocarditis Caused by Candida dubliniensis. J. Clin. Microbiol. 43: 3023-3026 [Abstract] [Full Text]  
  • Davis, L. E., Shields, C. E., Merz, W. G. (2005). Use of a Commercial Reagent Leads to Reduced Germ Tube Production by Candida dubliniensis. J. Clin. Microbiol. 43: 2465-2466 [Abstract] [Full Text]  
  • Pancholi, P., Park, S., Perlin, D., Kubin, C., Della-Latta, P. (2004). Molecular Characterization of Fluconazole Resistance in a Case of Candida albicans Ocular Infection. J. Clin. Microbiol. 42: 5938-5939 [Abstract] [Full Text]  
  • Marot-Leblond, A., Grimaud, L., David, S., Sullivan, D. J., Coleman, D. C., Ponton, J., Robert, R. (2004). Evaluation of a Rapid Immunochromatographic Assay for Identification of Candida albicans and Candida dubliniensis. J. Clin. Microbiol. 42: 4956-4960 [Abstract] [Full Text]  
  • Massonet, C., Van Eldere, J., Vaneechoutte, M., De Baere, T., Verhaegen, J., Lagrou, K. (2004). Comparison of VITEK 2 with ITS2-Fragment Length Polymorphism Analysis for Identification of Yeast Species. J. Clin. Microbiol. 42: 2209-2211 [Abstract] [Full Text]  
  • Al Mosaid, A., Sullivan, D. J., Coleman, D. C. (2003). Differentiation of Candida dubliniensis from Candida albicans on Pal's Agar. J. Clin. Microbiol. 41: 4787-4789 [Abstract] [Full Text]  
  • Fotedar, R., Al Hedaithy, S. S. A. (2003). Candida dubliniensis at a University Hospital in Saudi Arabia. J. Clin. Microbiol. 41: 1907-1911 [Abstract] [Full Text]  
  • Mosca, C. O., Moragues, M. D., Llovo, J., Al Mosaid, A., Coleman, D. C., Ponton, J. (2003). Casein Agar: a Useful Medium for Differentiating Candida dubliniensis from Candida albicans. J. Clin. Microbiol. 41: 1259-1262 [Abstract] [Full Text]  
  • Pfaller, M. A., Diekema, D. J., Messer, S. A., Boyken, L., Hollis, R. J., Jones, R. N. (2003). In Vitro Activities of Voriconazole, Posaconazole, and Four Licensed Systemic Antifungal Agents against Candida Species Infrequently Isolated from Blood. J. Clin. Microbiol. 41: 78-83 [Abstract] [Full Text]  
  • Yang, C. W., Barkham, T. M. S., Chan, F. Y., Wang, Y. (2003). Prevalence of Candida Species, Including Candida dubliniensis, in Singapore. J. Clin. Microbiol. 41: 472-474 [Abstract] [Full Text]  
  • Sancak, B., Rex, J. H., Paetznick, V., Chen, E., Rodriguez, J. (2003). Evaluation of a Method for Identification of Candida dubliniensis Bloodstream Isolates. J. Clin. Microbiol. 41: 489-491 [Abstract] [Full Text]  
  • Selvarangan, R., Limaye, A. P., Cookson, B. T. (2002). Rapid Identification and Differentiation of Candida albicans and Candida dubliniensis by Capillary-Based Amplification and Fluorescent Probe Hybridization. J. Clin. Microbiol. 40: 4308-4312 [Abstract] [Full Text]  
  • Gee, S. F., Joly, S., Soll, D. R., Meis, J. F. G. M., Verweij, P. E., Polacheck, I., Sullivan, D. J., Coleman, D. C. (2002). Identification of Four Distinct Genotypes of Candida dubliniensis and Detection of Microevolution In Vitro and In Vivo. J. Clin. Microbiol. 40: 556-574 [Abstract] [Full Text]  
  • Al Mosaid, A., Sullivan, D., Salkin, I. F., Shanley, D., Coleman, D. C. (2001). Differentiation of Candida dubliniensis from Candida albicans on Staib Agar and Caffeic Acid-Ferric Citrate Agar. J. Clin. Microbiol. 39: 323-327 [Abstract] [Full Text]  
  • Slifkin, M. (2000). Tween 80 Opacity Test Responses of Various Candida Species. J. Clin. Microbiol. 38: 4626-4628 [Abstract] [Full Text]  
  • Peltroche-Llacsahuanga, H., Schmidt, S., Seibold, M., Lütticken, R., Haase, G. (2000). Differentiation between Candida dubliniensis and Candida albicans by Fatty Acid Methyl Ester Analysis Using Gas-Liquid Chromatography. J. Clin. Microbiol. 38: 3696-3704 [Abstract] [Full Text]  
  • Park, S., Wong, M., Marras, S. A. E., Cross, E. W., Kiehn, T. E., Chaturvedi, V., Tyagi, S., Perlin, D. S. (2000). Rapid Identification of Candida dubliniensis Using a Species-Specific Molecular Beacon. J. Clin. Microbiol. 38: 2829-2836 [Abstract] [Full Text]  
  • Meis, J. F. G. M., Verduyn Lunel, F. M., Verweij, P. E., Voss;, A., Coleman, D. (2000). One-Year Prevalence of Candida dublinienis in a Dutch University Hospital. J. Clin. Microbiol. 38: 3139-3140 [Full Text]  
  • Jabra-Rizk, M. A., Falkler, W. A. Jr., Merz, W. G., Baqui, A. A. M. A., Kelley, J. I., Meiller, T. F. (2000). Retrospective Identification and Characterization of Candida dubliniensis Isolates among Candida albicans Clinical Laboratory Isolates from Human Immunodeficiency Virus (HIV)-Infected and Non-HIV-Infected Individuals. J. Clin. Microbiol. 38: 2423-2426 [Abstract] [Full Text]  
  • Polacheck, I., Strahilevitz, J., Sullivan, D., Donnelly, S., Salkin, I. F., Coleman, D. C. (2000). Recovery of Candida dubliniensis from Non-Human Immunodeficiency Virus-Infected Patients in Israel. J. Clin. Microbiol. 38: 170-174 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»