This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Ijdo, J. W.
Right arrow Articles by Fikrig, E.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Ijdo, J. W.
Right arrow Articles by Fikrig, E.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, November 1999, p. 3540-3544, Vol. 37, No. 11
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Serodiagnosis of Human Granulocytic Ehrlichiosis by a Recombinant HGE-44-Based Enzyme-Linked Immunosorbent Assay

Jacob W. Ijdo,1 Caiyun Wu,1 Louis A. Magnarelli,2 and Erol Fikrig1,*

Section of Rheumatology, Department of Internal Medicine, Yale University School of Medicine,1 and Connecticut Agricultural Experiment Station,2 New Haven, Connecticut

Received 11 May 1999/Returned for modification 30 June 1999/Accepted 22 July 1999

Current antibody testing for human granulocytic ehrlichiosis relies predominantly on indirect fluorescent-antibody assays and immunoblot analysis. Shortcomings of these techniques include high cost and variability of test results associated with the use of different strains of antigens derived from either horses or cultured HL-60 cells. We used recombinant protein HGE-44, expressed and purified as a maltose-binding protein (MBP) fusion peptide, as an antigen in a polyvalent enzyme-linked immunosorbent assay (ELISA). Fifty-five normal serum samples from healthy humans served as a reference to establish cutoff levels. Thirty-three of 38 HGE patient serum samples (87%), previously confirmed by positive whole-cell immunoblotting, reacted positively in the recombinant ELISA. In specificity analyses, serum samples from patients with Lyme disease, syphilis, rheumatoid arthritis, and human monocytic ehrlichiosis (HME) did not react with HGE-44-MBP antigen, except for one sample (specificity, 98%). We conclude that recombinant HGE-44 antigen is a suitable antigen in an ELISA for the laboratory diagnosis and epidemiological study of HGE.

* Corresponding author. Mailing address: 608 Laboratory of Clinical Investigation, Section of Rheumatology, Department of Internal Medicine, Yale University School of Medicine, 333 Cedar St., New Haven, CT 06520-8031. Phone: (203) 785-2453. Fax: (203) 785-7053. E-mail: efb{at}emailmed.yale.edu.

Journal of Clinical Microbiology, November 1999, p. 3540-3544, Vol. 37, No. 11
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Sarkar, M., Troese, M. J., Kearns, S. A., Yang, T., Reneer, D. V., Carlyon, J. A. (2008). Anaplasma phagocytophilum MSP2(P44)-18 Predominates and Is Modified into Multiple Isoforms in Human Myeloid Cells. Infect. Immun. 76: 2090-2098 [Abstract] [Full Text]  
  • KRAUSE, P. J., FOLEY, D. T., BURKE, G. S., CHRISTIANSON, D., CLOSTER, L., SPIELMAN, A., THE TICK-BORNE DISEASE STUDY GROUP, (2006). REINFECTION AND RELAPSE IN EARLY LYME DISEASE. Am J Trop Med Hyg 75: 1090-1094 [Abstract] [Full Text]  
  • Magnarelli, L. A., Stafford, K. C. III, IJdo, J. W., Fikrig, E. (2006). ANTIBODIES TO WHOLE-CELL OR RECOMBINANT ANTIGENS OF BORRELIA BURGDORFERI, ANAPLASMA PHAGOCYTOPHILUM, AND BABESIA MICROTI IN WHITE-FOOTED MICE. J Wildl Dis 42: 732-738 [Abstract] [Full Text]  
  • Wang, X., Kikuchi, T., Rikihisa, Y. (2006). Two Monoclonal Antibodies with Defined Epitopes of P44 Major Surface Proteins Neutralize Anaplasma phagocytophilum by Distinct Mechanisms. Infect. Immun. 74: 1873-1882 [Abstract] [Full Text]  
  • IJdo, J. W., Mueller, A. C. (2004). Neutrophil NADPH Oxidase Is Reduced at the Anaplasma phagocytophilum Phagosome. Infect. Immun. 72: 5392-5401 [Abstract] [Full Text]  
  • Magnarelli, L. A., IJdo, J. W., Ramakrishnan, U., Henderson, D. W., Stafford, K. C. III, Fikrig, E. (2004). USE OF RECOMBINANT ANTIGENS OF BORRELIA BURGDORFERI AND ANAPLASMA PHAGOCYTOPHILUM IN ENZYME-LINKED IMMUNOSORBENT ASSAYS TO DETECT ANTIBODIES IN WHITE-TAILED DEER. J Wildl Dis 40: 249-258 [Abstract] [Full Text]  
  • Salazar, J. C., Pope, C. D., Sellati, T. J., Feder, H. M. Jr, Kiely, T. G., Dardick, K. R., Buckman, R. L., Moore, M. W., Caimano, M. J., Pope, J. G., Krause, P. J., Radolf, J. D. (2003). Coevolution of Markers of Innate and Adaptive Immunity in Skin and Peripheral Blood of Patients with Erythema Migrans. J. Immunol. 171: 2660-2670 [Abstract] [Full Text]  
  • Paddock, C. D., Childs, J. E. (2003). Ehrlichia chaffeensis: a Prototypical Emerging Pathogen. Clin. Microbiol. Rev. 16: 37-64 [Abstract] [Full Text]  
  • IJdo, J. W., Wu, C., Telford III, S. R., Fikrig, E. (2002). Differential Expression of the p44 Gene Family in the Agent of Human Granulocytic Ehrlichiosis. Infect. Immun. 70: 5295-5298 [Abstract] [Full Text]  
  • MAGNARELLI, L. A., IJDO, J. W., SHERMAN, B. A., BUSHMICH, S. L., LEVY, S. A., FIKRIG, E. (2002). Antibodies to granulocytic ehrlichiae in cattle from Connecticut. J Med Microbiol 51: 326-331 [Abstract] [Full Text]  
  • Unver, A., Felek, S., Paddock, C. D., Zhi, N., Horowitz, H. W., Wormser, G. P., Cullman, L. C., Rikihisa, Y. (2001). Western Blot Analysis of Sera Reactive to Human Monocytic Ehrlichiosis and Human Granulocytic Ehrlichiosis Agents. J. Clin. Microbiol. 39: 3982-3986 [Abstract] [Full Text]  
  • Akkoyunlu, M., Malawista, S. E., Anguita, J., Fikrig, E. (2001). Exploitation of Interleukin-8-Induced Neutrophil Chemotaxis by the Agent of Human Granulocytic Ehrlichiosis. Infect. Immun. 69: 5577-5588 [Abstract] [Full Text]  
  • De Martino, S. J., Carlyon, J. A., Fikrig, E. (2001). Coinfection with Borrelia burgdorferi and the Agent of Human Granulocytic Ehrlichiosis. NEJM 345: 150-151 [Full Text]  
  • Lodes, M. J., Mohamath, R., Reynolds, L. D., McNeill, P., Kolbert, C. P., Bruinsma, E. S., Benson, D. R., Hofmeister, E., Reed, S. G., Houghton, R. L., Persing, D. H. (2001). Serodiagnosis of Human Granulocytic Ehrlichiosis by Using Novel Combinations of Immunoreactive Recombinant Proteins. J. Clin. Microbiol. 39: 2466-2476 [Abstract] [Full Text]  
  • Alleman, A. R., Barbet, A. F., Bowie, M. V., Sorenson, H. L., Wong, S. J., Bélanger, M. (2000). Expression of a Gene Encoding the Major Antigenic Protein 2 Homolog of Ehrlichia chaffeensis and Potential Application for Serodiagnosis. J. Clin. Microbiol. 38: 3705-3709 [Abstract] [Full Text]  
  • Tajima, T., Zhi, N., Lin, Q., Rikihisa, Y., Horowitz, H. W., Ralfalli, J., Wormser, G. P., Hechemy, K. E. (2000). Comparison of Two Recombinant Major Outer Membrane Proteins of the Human Granulocytic Ehrlichiosis Agent for Use in an Enzyme-Linked Immunosorbent Assay. CVI 7: 652-657 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»