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Journal of Clinical Microbiology, November 1999, p. 3612-3614, Vol. 37, No. 11
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Comparison of the Quantiplex Version 3.0 Assay and a Sensitized Amplicor Monitor Assay for Measurement of Human Immunodeficiency Virus Type 1 RNA Levels in Plasma Samples

Helene C. Highbarger,1 W. Gregory Alvord,2 Min Kang Jiang,1 Akram S. Shah,1 Julia A. Metcalf,3 H. Clifford Lane,3 and Robin L. Dewar1,*

Virus Isolation Laboratory, Clinical Services Program, Science Applications International Corporation---Frederick,1 and Data Management Services, Inc.,2 National Cancer Institute-Frederick Cancer Research and Development Center, Frederick, Maryland 21702, and Laboratory of Immunoregulation, National Institute of Allergy and Infectious Diseases, Bethesda, Maryland 208923

Received 23 March 1999/Returned for modification 13 May 1999/Accepted 23 July 1999

This study evaluated correlation and agreement between version 3 of the Quantiplex human immunodeficiency virus type 1 (HIV-1) RNA assay (v3 branched DNA [bDNA]) and a sensitized Amplicor HIV-1 Monitor assay (reverse transcription [RT]-PCR) for the measurement of HIV RNA. Three hundred eighteen samples from 59 randomly selected, HIV-1-seropositive persons on various drug protocols from the National Institute of Allergy and Infectious Diseases HIV outpatient clinic were studied. The results indicate that v3 bDNA and RT-PCR are highly correlated (r = 0.98) and are in good agreement (mean difference in log10 copies/ml ± 2 standard deviations = 0.072 ± 0.371). The relationship between values obtained by both assays is given by the following equation: log10v3 bDNA = -0.0915 + 1.0052 · log10RT-PCR. This represents a 1.026-fold difference between log10RT-PCR values and log10v3 bDNA values.

* Corresponding author. Mailing address: Virus Isolation Laboratory, Clinical Services Program, SAIC---Frederick, NCI-FCRDC, Frederick, MD 21702. Phone: (301) 846-1910. Fax: (301) 846-6762. E-mail: rdewar{at}nih.gov.

Journal of Clinical Microbiology, November 1999, p. 3612-3614, Vol. 37, No. 11
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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