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Journal of Clinical Microbiology, November 1999, p. 3722-3724, Vol. 37, No. 11
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Isolation of Rickettsia prowazekii from Blood by Shell Vial Cell Culture

Marie-Laure Birg, Bernard La Scola, Véronique Roux, Philippe Brouqui, and Didier Raoult*

Unité des Rickettsies, CNRS UPRESA 6020, Faculté de Médecine, 13385 Marseille Cedex 05, France

Received 1 April 1999/Returned for modification 13 May 1999/Accepted 23 July 1999

A blood sample from a patient who returned from Algeria with a fever inoculated on human embryonic lung fibroblasts by the shell vial cell culture technique led to the recovery of Rickettsia prowazekii. The last clinical strain was isolated 30 years ago. Shell vial cell culture is a versatile method that could replace the classic animal and/or embryonated egg inoculation.

* Corresponding author. Mailing address: Unité des Rickettsies, CNRS UPRESA 6020, Faculté de Médecine, 27 Boulevard Jean Moulin, 13385 Marseille Cedex 05, France. Phone: 33.4.91.38.55.17. Fax: 33.4.91.83.03.90. E-mail: Didier.Raoult{at}medecine.univ-mrs.fr.

Journal of Clinical Microbiology, November 1999, p. 3722-3724, Vol. 37, No. 11
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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