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Journal of Clinical Microbiology, November 1999, p. 3746-3748, Vol. 37, No. 11
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Novel Method for Rapid Determination of Clarithromycin Sensitivity in Helicobacter pylori

J. R. Gibson,1 N. A. Saunders,2 B. Burke,1 and R. J. Owen1,*

Helicobacter Reference Unit, Laboratory of Enteric Pathogens,1 and Molecular Biology Unit, Virus Reference Division,2 Central Public Health Laboratory, London NW9 5HT, United Kingdom

Received 30 April 1999/Returned for modification 9 July 1999/Accepted 9 August 1999

A novel PCR-hybridization assay, performed in single closed capillaries, was developed to detect clarithromycin resistance-associated gene mutations in Helicobacter pylori. Mutations were detected by thermal analysis in 33 of 34 (97%) resistant isolates but not in 66 isolates determined to be sensitive by conventional antibiotic assays. The method was rapid and reproducible, and it reduced PCR product contamination risk.

* Corresponding author. Mailing address: Helicobacter Reference Unit, Laboratory of Enteric Pathogens, Central Public Health Laboratory, 61 Colindale Ave., London NW9 5HT, United Kingdom. Phone: (44) 181-2004400. Fax: (44) 181-9059929. E-mail: rowen{at}phls.nhs.uk.

Journal of Clinical Microbiology, November 1999, p. 3746-3748, Vol. 37, No. 11
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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