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Journal of Clinical Microbiology, November 1999, p. 3767-3768, Vol. 37, No. 11
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

PCR Assay for Detecting Porcine Cytomegalovirus

Andre L. Hamel, Lihua Lin, Cheryl Sachvie, Elsie Grudeski, and Gopi P. S. Nayar*

Virology Laboratory, Veterinary Services, Manitoba Agriculture, Winnipeg, Manitoba, Canada R3T 5S6

Received 16 June 1999/Returned for modification 12 July 1999/Accepted 19 July 1999

This is the first published report of a PCR assay for detecting porcine cytomegalovirus (PCMV), the causative agent of inclusion body rhinitis in pigs. The DNA to be tested was extracted directly from lungs and nasal scrapings of pigs with various clinical syndromes. Fifty-nine percent (74 of 126) of tested pigs with various clinical syndromes were found to be PCR positive for PCMV. It is hoped that veterinary diagnostic laboratories will benefit by using this PCR assay for routine testing and surveillance of PCMV in pigs.

* Corresponding author. Mailing address: Virology Laboratory, Veterinary Services, Manitoba Agriculture, 545 University Crescent, Winnipeg, Manitoba, Canada R3T 5S6. Phone: (204) 945-7643. Fax: (204) 945-8062. E-mail: gnayar{at}agr.gov.mb.ca.

Journal of Clinical Microbiology, November 1999, p. 3767-3768, Vol. 37, No. 11
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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