This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Yuen, L. K. W.
Right arrow Articles by Coloe, P. J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Yuen, L. K. W.
Right arrow Articles by Coloe, P. J.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, December 1999, p. 3844-3850, Vol. 37, No. 12
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Bacteriological and Molecular Analysis of Rifampin-Resistant Mycobacterium tuberculosis Strains Isolated in Australia

Lilly K. W. Yuen,1,* David Leslie,2 and Peter J. Coloe3

Victorian Infectious Diseases Reference Laboratory, North Melbourne, 3051,1 4 The Avenue, Balaclava, 3183,2 and Applied Biology and Biotechnology Department, RMIT University, Melbourne, 3000,3 Australia

Received 24 May 1999/Returned for modification 30 June 1999/Accepted 20 August 1999

To develop a better understanding of the epidemiology and molecular biology of rifampin-resistant Mycobacterium tuberculosis strains in Australia, 50 clinical isolates (33 rifampin-resistant and 17 rifampin-sensitive strains) cultured between 1990 and 1997 were analyzed by a number of bacteriological and molecular techniques. Examination of the drug resistance profiles of the 33 rifampin-resistant isolates revealed that 91% were resistant to rifampin in combination with resistance to isoniazid, 88% were resistant to rifampin on first isolation, and 81% showed cross-resistance with rifabutin. On the basis of the demographic data provided for the patients infected with the rifampin-resistant strains, 90% of the patients were born overseas. Of these patients, 64% developed clinical symptoms within 5 years of residence in Australia. On a molecular level, analysis of the rpoB gene revealed that 97% of the rifampin-resistant isolates had missense mutations within a conserved region of the gene, and eight types of missense mutations were detected. Of the 31 rifampin-resistant isolates that were typed by restriction fragment length polymorphism (RFLP) analysis, 28 distinct patterns were obtained by RFLP analysis with IS6110, and three clusters of genetically related isolates were identified. All isolates within the clusters were from patients who were born overseas and who had the same country of origin. The results from this study provide an overview of the current situation of rifampin resistance in Australia and can serve as a basis for continued monitoring of drug-resistant M. tuberculosis strains isolated within the country.

* Corresponding author. Mailing address: Victorian Infectious Diseases Reference Laboratory, 10 Wreckyn St., North Melbourne, 3051, Australia. Phone: 61-3-9342-2618. Fax: 61-3-9342-2665. E-mail: lilly.yuen{at}nwhcn.org.au.

Journal of Clinical Microbiology, December 1999, p. 3844-3850, Vol. 37, No. 12
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Van Deun, A., Barrera, L., Bastian, I., Fattorini, L., Hoffmann, H., Kam, K. M., Rigouts, L., Rusch-Gerdes, S., Wright, A. (2009). Mycobacterium tuberculosis Strains with Highly Discordant Rifampin Susceptibility Test Results. J. Clin. Microbiol. 47: 3501-3506 [Abstract] [Full Text]  
  • McCammon, M. T., Gillette, J. S., Thomas, D. P., Ramaswamy, S. V., Graviss, E. A., Kreiswirth, B. N., Vijg, J., Quitugua, T. N. (2005). Detection of rpoB Mutations Associated with Rifampin Resistance in Mycobacterium tuberculosis Using Denaturing Gradient Gel Electrophoresis. Antimicrob. Agents Chemother. 49: 2200-2209 [Abstract] [Full Text]  
  • O'Sullivan, D. M., McHugh, T. D., Gillespie, S. H. (2005). Analysis of rpoB and pncA mutations in the published literature: an insight into the role of oxidative stress in Mycobacterium tuberculosis evolution?. J Antimicrob Chemother 55: 674-679 [Abstract] [Full Text]  
  • Jou, R., Chen, H.-Y., Chiang, C.-Y., Yu, M.-C., Su, I.-J. (2005). Genetic Diversity of Multidrug-Resistant Mycobacterium tuberculosis Isolates and Identification of 11 Novel rpoB Alleles in Taiwan. J. Clin. Microbiol. 43: 1390-1394 [Abstract] [Full Text]  
  • Yam, W. C., Tam, C. M., Leung, C. C., Tong, H. L., Chan, K. H., Leung, E. T. Y., Wong, K. C., Yew, W. W., Seto, W. H., Yuen, K. Y., Ho, P. L. (2004). Direct Detection of Rifampin-Resistant Mycobacterium tuberculosis in Respiratory Specimens by PCR-DNA Sequencing. J. Clin. Microbiol. 42: 4438-4443 [Abstract] [Full Text]  
  • Mariam, D. H., Mengistu, Y., Hoffner, S. E., Andersson, D. I. (2004). Effect of rpoB Mutations Conferring Rifampin Resistance on Fitness of Mycobacterium tuberculosis. Antimicrob. Agents Chemother. 48: 1289-1294 [Abstract] [Full Text]  
  • Yue, J., Shi, W., Xie, J., Li, Y., Zeng, E., Wang, H. (2003). Mutations in the rpoB Gene of Multidrug-Resistant Mycobacterium tuberculosis Isolates from China. J. Clin. Microbiol. 41: 2209-2212 [Abstract] [Full Text]  
  • Fan, X.-Y., Hu, Z.-Y., Xu, F.-H., Yan, Z.-Q., Guo, S.-Q., Li, Z.-M. (2003). Rapid Detection of rpoB Gene Mutations in Rifampin-Resistant Mycobacterium tuberculosis Isolates in Shanghai by Using the Amplification Refractory Mutation System. J. Clin. Microbiol. 41: 993-997 [Abstract] [Full Text]  
  • Cavusoglu, C., Hilmioglu, S., Guneri, S., Bilgic, A. (2002). Characterization of rpoB Mutations in Rifampin-Resistant Clinical Isolates of Mycobacterium tuberculosis from Turkey by DNA Sequencing and Line Probe Assay. J. Clin. Microbiol. 40: 4435-4438 [Abstract] [Full Text]  
  • Bartfai, Z., Somoskovi, A., Kodmon, C., Szabo, N., Puskas, E., Kosztolanyi, L., Farago, E., Mester, J., Parsons, L. M., Salfinger, M. (2001). Molecular Characterization of Rifampin-Resistant Isolates of Mycobacterium tuberculosis from Hungary by DNA Sequencing and the Line Probe Assay. J. Clin. Microbiol. 39: 3736-3739 [Abstract] [Full Text]  
  • Mani, C., Selvakumar, N., Narayanan, S., Narayanan, P. R. (2001). Mutations in the rpoB Gene of Multidrug-Resistant Mycobacterium tuberculosis Clinical Isolates from India. J. Clin. Microbiol. 39: 2987-2990 [Abstract] [Full Text]  
  • Serr, A., Koenig, B. F., Heep, M., Nielsen, K., Bangsborg, J. M. (2001). Update on Rifampin Resistance in the Legionellaceae. Antimicrob. Agents Chemother. 45: 2181-2182 [Full Text]  
  • Heep, M., Brandstätter, B., Rieger, U., Lehn, N., Richter, E., Rüsch-Gerdes, S., Niemann, S. (2001). Frequency of rpoB Mutations Inside and Outside the Cluster I Region in Rifampin-Resistant Clinical Mycobacterium tuberculosis Isolates. J. Clin. Microbiol. 39: 107-110 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»