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Journal of Clinical Microbiology, December 1999, p. 3879-3882, Vol. 37, No. 12
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Determination of Bovine Rotavirus G and P Serotypes in Italy by PCR

E. Falcone,1 M. Tarantino,1 L. Di Trani,1 P. Cordioli,2 A. Lavazza,2 and M. Tollis1,*

Istituto Superiore di Sanità, 00161 Rome,1 and Istituto Zooprofilattico Sperimentale della Lombardia e dell'Emilia Romagna, Brescia,2 Italy

Received 28 December 1998/Returned for modification 31 March 1999/Accepted 7 July 1999

Determination of the G and P serotypes of group A bovine rotaviruses from 149 samples of feces or intestinal contents collected from calves showing clinical signs of neonatal diarrhea was performed by a nested reverse transcription-PCR typing assay. The G6 serotype was the most prevalent, accounting for viruses in 55.7% of the samples; viruses of the G10 and G8 serotypes were found in 34.9 and 4.7% of the samples, respectively. The virus in one sample (0.7%) was not classified due to concomitant infection with G6 and G8 strains, whereas viruses in six samples (4.0%) could not be characterized with any of the three G serotype-specific primers selected for the present study. When examined for their P-serotype specificities, viruses in 55 and 42.3% of the samples were characterized as P[11] and P[5], respectively, no P[1] serotype was identified, and viruses in 2.7% of the samples could not be classified due to multiple reactivity with both P[5]- and P[11]-specific primers. Various combinations of G and P serotypes were observed, the most frequent being G6,P[5] (38.3%), G10,P[11] (31.5%), and G6,P[11] (15.4%). The results of the present study, while contributing to a better understanding of the epidemiology of bovine rotaviruses in Italy, address the relevance of serotype specificity with regard to the constancy of the quality of bovine rotavirus vaccines under different field conditions.

* Corresponding author. Mailing address: Istituto Superiore di Sanità, Viale R. Elena 299, 00161 Rome, Italy. Phone: 0039649902673. Fax: 0039649387077. E-mail: m.tollis{at}iss.it.

Journal of Clinical Microbiology, December 1999, p. 3879-3882, Vol. 37, No. 12
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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