This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Covalciuc, K. A.
Right arrow Articles by Carlson, C. A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Covalciuc, K. A.
Right arrow Articles by Carlson, C. A.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, December 1999, p. 3971-3974, Vol. 37, No. 12
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Comparison of Four Clinical Specimen Types for Detection of Influenza A and B Viruses by Optical Immunoassay (FLU OIA Test) and Cell Culture Methods

Kristi A. Covalciuc, Kenneth H. Webb,* and Curtis A. Carlson

BioStar, Inc., Boulder, Colorado 80301

Received 24 May 1999/Returned for modification 21 July 1999/Accepted 16 September 1999

Although laboratory diagnosis of respiratory viruses has been widely studied, there is a relative insufficiency of literature examining the impact of specimen type on the laboratory diagnosis of influenza A and B. In a clinical study comparing the FLU OIA test with 14-day cell culture, clinical specimens from nasopharyngeal swabs, throat swabs, nasal aspirates, and sputum were obtained from patients experiencing influenza-like symptoms. A total of 404 clinical specimens were collected from 184 patients. Patients were defined as influenza positive if the viral culture of a specimen from any sample site was positive. Patients were defined as influenza negative if the viral cultures of specimens from all sample sites were negative. By this gold standard, culture and FLU OIA test results for each sample type were compared. For each of the four specimen types, the viral culture and FLU OIA test demonstrated equal abilities to detect the presence of influenza A or B virus or viral antigen. Sputum and nasal aspirate samples were the most predictive of influenza virus infection. Throat swabs were the least predictive of influenza virus infection, with both tests failing to detect influenza virus in nearly 50% of the throat samples studied.

* Corresponding author. Mailing address: BioStar, Inc., 6655 Lookout Rd., Boulder, CO 80301-3371. Phone: (800) 637-3717, ext. 652. Fax: (303) 530-6627. E-mail: k_webb{at}biostar.com.

Journal of Clinical Microbiology, December 1999, p. 3971-3974, Vol. 37, No. 12
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Lieberman, D., Lieberman, D., Shimoni, A., Keren-Naus, A., Steinberg, R., Shemer-Avni, Y. (2009). Identification of Respiratory Viruses in Adults: Nasopharyngeal versus Oropharyngeal Sampling. J. Clin. Microbiol. 47: 3439-3443 [Abstract] [Full Text]  
  • Rouleau, I., Charest, H., Douville-Fradet, M., Skowronski, D. M., De Serres, G. (2009). Field Performance of a Rapid Diagnostic Test for Influenza in an Ambulatory Setting. J. Clin. Microbiol. 47: 2699-2703 [Abstract] [Full Text]  
  • Krief, W. I., Levine, D. A., Platt, S. L., Macias, C. G., Dayan, P. S., Zorc, J. J., Feffermann, N., Kuppermann, N., for the Multicenter RSV-SBI Study Group of the Ped, (2009). Influenza Virus Infection and the Risk of Serious Bacterial Infections in Young Febrile Infants. Pediatrics 124: 30-39 [Abstract] [Full Text]  
  • Loens, K., Van Heirstraeten, L., Malhotra-Kumar, S., Goossens, H., Ieven, M. (2009). Optimal Sampling Sites and Methods for Detection of Pathogens Possibly Causing Community-Acquired Lower Respiratory Tract Infections. J. Clin. Microbiol. 47: 21-31 [Full Text]  
  • Walsh, P., Overmyer, C. L., Pham, K., Michaelson, S., Gofman, L., DeSalvia, L., Tran, T., Gonzalez, D., Pusavat, J., Feola, M., Iacono, K. T., Mordechai, E., Adelson, M. E. (2008). Comparison of Respiratory Virus Detection Rates for Infants and Toddlers by Use of Flocked Swabs, Saline Aspirates, and Saline Aspirates Mixed in Universal Transport Medium for Room Temperature Storage and Shipping. J. Clin. Microbiol. 46: 2374-2376 [Abstract] [Full Text]  
  • Agoritsas, K., Mack, K., Bonsu, B. K., Goodman, D., Salamon, D., Marcon, M. J. (2006). Evaluation of the Quidel QuickVue Test for Detection of Influenza A and B Viruses in the Pediatric Emergency Medicine Setting by Use of Three Specimen Collection Methods.. J. Clin. Microbiol. 44: 2638-2641 [Abstract] [Full Text]  
  • Fader, R. C. (2005). Comparison of the Binax NOW Flu A Enzyme Immunochromatographic Assay and R-Mix Shell Vial Culture for the 2003-2004 Influenza Season. J. Clin. Microbiol. 43: 6133-6135 [Abstract] [Full Text]  
  • Strom, C. M., Clark, D. D., Hantash, F. M., Rea, L., Anderson, B., Maul, D., Huang, D., Traul, D., Chen Tubman, C., Garcia, R., Hess, P. P., Wang, H., Crossley, B., Woodruff, E., Chen, R., Killeen, M., Sun, W., Beer, J., Avens, H., Polisky, B., Jenison, R. D. (2004). Direct Visualization of Cystic Fibrosis Transmembrane Regulator Mutations in the Clinical Laboratory Setting. Clin. Chem. 50: 836-845 [Abstract] [Full Text]  
  • Ruest, A., Michaud, S., Deslandes, S., Frost, E. H. (2003). Comparison of the Directigen Flu A+B Test, the QuickVue Influenza Test, and Clinical Case Definition to Viral Culture and Reverse Transcription-PCR for Rapid Diagnosis of Influenza Virus Infection. J. Clin. Microbiol. 41: 3487-3493 [Abstract] [Full Text]  
  • Bonner, A. B., Monroe, K. W., Talley, L. I., Klasner, A. E., Kimberlin, D. W. (2003). Impact of the Rapid Diagnosis of Influenza on Physician Decision-Making and Patient Management in the Pediatric Emergency Department: Results of a Randomized, Prospective, Controlled Trial. Pediatrics 112: 363-367 [Abstract] [Full Text]  
  • Henrickson, K. J. (2003). Parainfluenza Viruses. Clin. Microbiol. Rev. 16: 242-264 [Abstract] [Full Text]  
  • Heikkinen, T., Marttila, J., Salmi, A. A., Ruuskanen, O. (2002). Nasal Swab versus Nasopharyngeal Aspirate for Isolation of Respiratory Viruses. J. Clin. Microbiol. 40: 4337-4339 [Abstract] [Full Text]  
  • Reina, J., Padilla, E., Alonso, F., Ruiz de Gopegui, E., Munar, M., Mari, M. (2002). Evaluation of a New Dot Blot Enzyme Immunoassay (Directigen Flu A+B) for Simultaneous and Differential Detection of Influenza A and B Virus Antigens from Respiratory Samples. J. Clin. Microbiol. 40: 3515-3517 [Abstract] [Full Text]  
  • Poehling, K. A., Griffin, M. R., Dittus, R. S., Tang, Y.-W., Holland, K., Li, H., Edwards, K. M. (2002). Bedside Diagnosis of Influenzavirus Infections in Hospitalized Children. Pediatrics 110: 83-88 [Abstract] [Full Text]  
  • Jenison, R., La, H., Haeberli, A., Ostroff, R., Polisky, B. (2001). Silicon-based Biosensors for Rapid Detection of Protein or Nucleic Acid Targets. Clin. Chem. 47: 1894-1900 [Abstract] [Full Text]  
  • Zambon, M., Hays, J., Webster, A., Newman, R., Keene, O. (2001). Diagnosis of Influenza in the Community: Relationship of Clinical Diagnosis to Confirmed Virological, Serologic, or Molecular Detection of Influenza. Arch Intern Med 161: 2116-2122 [Abstract] [Full Text]  
  • Boivin, G., Hardy, I., Kress, A. (2001). Evaluation of a Rapid Optical Immunoassay for Influenza Viruses (FLU OIA Test) in Comparison with Cell Culture and Reverse Transcription-PCR. J. Clin. Microbiol. 39: 730-732 [Abstract] [Full Text]  
  • Heikkinen, T., Salmi, A. A, Ruuskanen, O. (2001). Comparative study of nasopharyngeal aspirate and nasal swab specimens for detection of influenza. BMJ 322: 138-138 [Full Text]  
  • Herrmann, B., Larsson, C., Zweygberg, B. W. (2001). Simultaneous Detection and Typing of Influenza Viruses A and B by a Nested Reverse Transcription-PCR: Comparison to Virus Isolation and Antigen Detection by Immunofluorescence and Optical Immunoassay (FLU OIA). J. Clin. Microbiol. 39: 134-138 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»