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Journal of Clinical Microbiology, December 1999, p. 4150-4152, Vol. 37, No. 12
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Evaluation of Three Commercial Serological Tests with Different Methodologies To Assess Helicobacter pylori Infection

A. van der Ende,1,* R. W. M. van der Hulst,2,3,dagger P. Roorda,1 G. N. J. Tytgat,2 and J. Dankert1

Department of Medical Microbiology1 and Department of Gastroenterology,2 Academic Medical Center, Amsterdam, The Netherlands3

Received 19 April 1999/Returned for modification 21 August 1999/Accepted 7 September 1999

The sera of 142 Helicobacter pylori-positive and 32 H. pylori-negative patients were assessed by a desktop test (QuickVue), an enzyme-linked immunosorbent assay (ELISA) (HM-CAP), and a solid-phase, two-step chemiluminescent enzyme immunoassay (Immulite). These tests yielded sensitivities of 97, 97, and 91% and specificities of 97, 94, and 100%, respectively. In conclusion, the desktop test and the ELISA are more sensitive than the chemiluminescent enzyme immunoassay (P < 0.05). The chemiluminescent enzyme immunoassay has the advantage that it is fully automated.

* Corresponding author. Mailing address: Department of Medical Microbiology, Academic Medical Center, University of Amsterdam, P.O. Box 22700, 1100 DE Amsterdam, The Netherlands. Phone: 31-20-5664862. Fax: 31-20-6979271.

dagger Present address: Kennemer Gasthuis, Department of Internal Medicine, Gastroenterology, 2023 EA Haarlem, The Netherlands.

Journal of Clinical Microbiology, December 1999, p. 4150-4152, Vol. 37, No. 12
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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