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Journal of Clinical Microbiology, December 1999, p. 4179-4182, Vol. 37, No. 12
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Development and Evaluation of a Rapid Dipstick Assay for Serodiagnosis of Acute Human Brucellosis

Henk L. Smits,1,* M. A. Basahi,2 Ramon Díaz,3 T. Marrodan,3 James T. Douglas,4 Alice Rocha,5 Jaques Veerman,2 M. M. Zheludkov,6 Olav W. M. Witte,7 Joop de Jong,8 George C. Gussenhoven,1 Marga G. A. Goris,1 and Menno A. W. G. van der Hoorn1

Department of Biomedical Research, Royal Tropical Institute,1 and Médicins sans Frontières,8 Amsterdam, and Department of Microbiology, DLO-Institute for Animal Science and Health, Lelystad,7 The Netherlands; Yhoc Sana'a Medical Laboratories, Sana'a, Yemen2; Department of Clinical Microbiology, Universidad de Navarra, Pamplona, Spain3; Department of Microbiology, University of Hawaii, Honolulu, Hawaii4; Institute Nacional de Saude, Lisbon, Portugal5; and Gamaleya Research Institute of Epidemiology and Microbiology, Moscow, Russia6

Received 26 March 1999/Returned for modification 4 June 1999/Accepted 9 September 1999

A dipstick assay for the detection of brucella-specific immunoglobulin M antibodies was evaluated with 707 sera from 247 laboratory-confirmed brucellosis patients and 342 control sera from brucellosis-free individuals. These sera were collected from six different countries. The assay was found to be highly sensitive and specific. In addition, the test is easy to use and does not require specialized training or equipment, and the components are stable without a requirement for refrigeration. All of these factors make the test ideal for developing countries and rural settings.

* Corresponding author. Mailing address: Department of Biomedical Research, Royal Tropical Institute, Meibergdreef 39, 1105 AZ Amsterdam, The Netherlands. Phone: 31-20-5665470. Fax: 31-20-6971841. E-mail: H.Smits{at}kit.nl.

Journal of Clinical Microbiology, December 1999, p. 4179-4182, Vol. 37, No. 12
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Clavijo, E., Diaz, R., Anguita, A., Garcia, A., Pinedo, A., Smits, H. L. (2003). Comparison of a Dipstick Assay for Detection of Brucella-Specific Immunoglobulin M Antibodies with Other Tests for Serodiagnosis of Human Brucellosis. CVI 10: 612-615 [Abstract] [Full Text]  
  • Ismail, T. F., Smits, H., Wasfy, M. O., Malone, J. L., Fadeel, M. A., Mahoney, F. (2002). Evaluation of Dipstick Serologic Tests for Diagnosis of Brucellosis and Typhoid Fever in Egypt. J. Clin. Microbiol. 40: 3509-3511 [Abstract] [Full Text]  
  • MARRODAN, T., NENOVA-POLIAKOVA, R., RUBIO, M., ARIZA, J., CLAVIJO, E., SMITS, H.L., DIAZ, R. (2001). Evaluation of three methods to measure anti-Brucella IgM antibodies and interference of IgA in the interpretation of mercaptan-based tests. J Med Microbiol 50: 663-666 [Abstract] [Full Text]  
  • Orduña, A., Almaraz, A., Prado, A., Gutierrez, M. P., Garcia-Pascual, A., Dueñas, A., Cuervo, M., Abad, R., Hernández, B., Lorenzo, B., Bratos, M. A., Torres, A. R. (2000). Evaluation of an Immunocapture-Agglutination Test (Brucellacapt) for Serodiagnosis of Human Brucellosis. J. Clin. Microbiol. 38: 4000-4005 [Abstract] [Full Text]  


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