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Journal of Clinical Microbiology, February 1999, p. 380-385, Vol. 37, No. 2
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Pulsed-Field Gel Electrophoresis Is More Efficient than Ribotyping and Random Amplified Polymorphic DNA Analysis in Discrimination of Pasteurella haemolytica Strains

Angeli Kodjo,1,* Laurence Villard,1 Chantal Bizet,2 Jean-Louis Martel,3 Richard Sanchis,4 Evelyne Borges,1 Dominique Gauthier,5 Françoise Maurin,1 and Yves Richard1

Ecole Nationale Vétérinaire de Lyon, F-69280 Marcy l'Etoile,1 Collection Institut Pasteur BP 52, F-75724 Paris Cedex 15,2 CNEVA-Lyon, F-69342 Lyon cedex 07,3 CNEVA-Sophia Antipolis, F-06902 Sophia Antipolis Cedex,4 and Laboratoire Départemental d'Analyses, F-73000 Chambery,5 France

Received 26 May 1998/Returned for modification 7 August 1998/Accepted 14 October 1998

One hundred thirty-three strains of Pasteurella haemolytica of both biotypes (90 and 43 strains of biotypes A and T, respectively) and almost all the serotypes were subjected to ribotyping, random amplified polymorphic DNA (RAPD) analysis, and pulsed-field gel electrophoresis (PFGE) analysis for epidemiological purposes. A total of 15 patterns recorded as ribotypes HA to HO were found for the P. haemolytica biotype A strains, with ribotypes HA, HC, and HD being encountered most often (66 strains [74%]); and 20 ribotypes, designated HA' to HT', that were clearly distinct from those observed for biotype A strains were observed for strains of biotype T. RAPD analysis generated a total of 44 (designated Rp1 to Rp44) and 15 (designated Rp1' to Rp 15') unique RAPD patterns for biogroup A and biogroup T, respectively. Analysis of the data indicated that a given combined ribotype-RAPD pattern could be observed for biotype A strains of different serotypes, whatever the zoological or geographic origin, whereas this was not the case for biotype T strains. PFGE appeared to be more efficient in strain discrimination since selected strains from various zoological or geographical origins harboring the same ribotype-RAPD group were further separated into unique entities.

* Corresponding author. Mailing address: Ecole Nationale Vétérinaire de Lyon, BP 83, 1 Av. Bourgelat, F-69280 Marcy l'Etoile, France. Phone: 33 4 78 87 25 55. Fax: 33 4 78 87 25 94. E-mail: a.kodjo{at}vet-lyon.fr.

Journal of Clinical Microbiology, February 1999, p. 380-385, Vol. 37, No. 2
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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