This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Farrell, D. J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Farrell, D. J.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, February 1999, p. 386-390, Vol. 37, No. 2
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Evaluation of AMPLICOR Neisseria gonorrhoeae PCR Using cppB Nested PCR and 16S rRNA PCR

David J. Farrell*

Microbiology Section, Queensland Health Pathology Service Toowoomba Laboratory, Toowoomba, Queensland, Australia 4350

Received 18 August 1998/Returned for modification 24 September 1998/Accepted 10 November 1998

Certain strains of Neisseria subflava and Neisseria cinerea are known to produce false-positive results with the AMPLICOR Neisseria gonorrhoeae PCR (Roche Diagnostic Systems, Branchburg, N.J.). The analytical sensitivity and analytical specificity of three PCR tests were assessed with 3 geographically diverse N. gonorrhoeae strains and 30 non-N. gonorrhoeae Neisseria spp. The sensitivities of the in-house nested cppB gene and the 16S rRNA PCR methods were greater than that of the AMPLICOR N. gonorrhoeae PCR with purified DNA from all 3 N. gonorrhoeae strains. Six of 14 clinical strains of N. subflava (1 from a vaginal swab, 5 from respiratory sites) produced false-positive AMPLICOR N. gonorrhoeae PCR results and were negative by the two other PCR methods. When applied to 207 clinical specimens selected from a population with a high prevalence (~9%) of infection, the results for 15 of 96 (15.6%) AMPLICOR-positive specimens and 14 of 17 (82.3%) AMPLICOR-equivocal specimens were not confirmed by the more sensitive nested cppB PCR method. Only 2 of 94 (2.1%) of AMPLICOR N. gonorrhoeae PCR-negative specimens from the same population tested positive by the nested cppB method. These results suggest that for this population the AMPLICOR N. gonorrhoeae PCR test is suitable as a screening test only and all positive results should be confirmed by a PCR method that is more specific and at least as sensitive. This study also illustrates that caution should be used when introducing commercially available nucleic acid amplification-based diagnostic tests into the regimens of tests used for populations not previously tested with these products.

* Mailing address: Microbiology Section, Queensland Health Pathology Service Toowoomba Laboratory, Toowoomba, Queensland, Australia, 4350. Phone: 61 7 46316562. Fax: 61 7 46392279. E-mail: David_Farrell{at}health.qld.gov.au.

Journal of Clinical Microbiology, February 1999, p. 386-390, Vol. 37, No. 2
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Moncada, J., Schachter, J., Liska, S., Shayevich, C., Klausner, J. D. (2009). Evaluation of Self-Collected Glans and Rectal Swabs from Men Who Have Sex with Men for Detection of Chlamydia trachomatis and Neisseria gonorrhoeae by Use of Nucleic Acid Amplification Tests. J. Clin. Microbiol. 47: 1657-1662 [Abstract] [Full Text]  
  • Masek, B. J., Arora, N., Quinn, N., Aumakhan, B., Holden, J., Hardick, A., Agreda, P., Barnes, M., Gaydos, C. A. (2009). Performance of Three Nucleic Acid Amplification Tests for Detection of Chlamydia trachomatis and Neisseria gonorrhoeae by Use of Self-Collected Vaginal Swabs Obtained via an Internet-Based Screening Program. J. Clin. Microbiol. 47: 1663-1667 [Abstract] [Full Text]  
  • Koressaar, T., Joers, K., Remm, M. (2009). Automatic identification of species-specific repetitive DNA sequences and their utilization for detecting microbial organisms. Bioinformatics 25: 1349-1355 [Abstract] [Full Text]  
  • McIver, C. J., Rismanto, N., Smith, C., Naing, Z. W., Rayner, B., Lusk, M. J., Konecny, P., White, P. A., Rawlinson, W. D. (2009). Multiplex PCR Testing Detection of Higher-than-Expected Rates of Cervical Mycoplasma, Ureaplasma, and Trichomonas and Viral Agent Infections in Sexually Active Australian Women. J. Clin. Microbiol. 47: 1358-1363 [Abstract] [Full Text]  
  • Bachmann, L. H., Johnson, R. E., Cheng, H., Markowitz, L. E., Papp, J. R., Hook, E. W. III (2009). Nucleic Acid Amplification Tests for Diagnosis of Neisseria gonorrhoeae Oropharyngeal Infections. J. Clin. Microbiol. 47: 902-907 [Abstract] [Full Text]  
  • Moncada, J., Donegan, E., Schachter, J. (2008). Evaluation of CDC-Recommended Approaches for Confirmatory Testing of Positive Neisseria gonorrhoeae Nucleic Acid Amplification Test Results. J. Clin. Microbiol. 46: 1614-1619 [Abstract] [Full Text]  
  • Peter-Getzlaff, S., Luethy, J., Springer, B., Kaul, K. L., Mangold, K. A. (2007). Diagnostic Value of Molecular Confirmation Assays for Neisseria gonorrhoeae. J. Clin. Microbiol. 45: 3856-3858 [Full Text]  
  • Munson, E., Boyd, V., Czarnecka, J., Griep, J., Lund, B., Schaal, N., Hryciuk, J. E. (2007). Evaluation of Gen-Probe APTIMA-Based Neisseria gonorrhoeae and Chlamydia trachomatis Confirmatory Testing in a Metropolitan Setting of High Disease Prevalence. J. Clin. Microbiol. 45: 2793-2797 [Abstract] [Full Text]  
  • Mangold, K. A., Regner, M., Tajuddin, M., Tajuddin, A. M., Jennings, L., Du, H., Kaul, K. L. (2007). Neisseria Species Identification Assay for the Confirmation of Neisseria gonorrhoeae-Positive Results of the COBAS Amplicor PCR. J. Clin. Microbiol. 45: 1403-1409 [Abstract] [Full Text]  
  • Marshall, R., Chernesky, M., Jang, D., Hook, E. W., Cartwright, C. P., Howell-Adams, B., Ho, S., Welk, J., Lai-Zhang, J., Brashear, J., Diedrich, B., Otis, K., Webb, E., Robinson, J., Yu, H. (2007). Characteristics of the m2000 Automated Sample Preparation and Multiplex Real-Time PCR System for Detection of Chlamydia trachomatis and Neisseria gonorrhoeae. J. Clin. Microbiol. 45: 747-751 [Abstract] [Full Text]  
  • Alary, M, Gbenafa-Agossa, C, Aina, G, Ndour, M, Labbe, A C, Fortin, D, Steele, M, Peeling, R W (2006). Evaluation of a rapid point-of-care test for the detection of gonococcal infection among female sex workers in Benin. Sex. Transm. Infect. 82: v29-v32 [Abstract] [Full Text]  
  • Hjelmevoll, S. O., Olsen, M. E., Sollid, J. U. E., Haaheim, H., Unemo, M., Skogen, V. (2006). A Fast Real-Time Polymerase Chain Reaction Method for Sensitive and Specific Detection of the Neisseria gonorrhoeae porA Pseudogene. J. Mol. Diagn. 8: 574-581 [Abstract] [Full Text]  
  • Du, Y., Arvidson, C. G. (2006). RpoH Mediates the Expression of Some, but Not All, Genes Induced in Neisseria gonorrhoeae Adherent to Epithelial Cells.. Infect. Immun. 74: 2767-2776 [Abstract] [Full Text]  
  • Whiley, D. M., Tapsall, J. W., Sloots, T. P. (2006). Nucleic Acid Amplification Testing for Neisseria gonorrhoeae: An Ongoing Challenge. J. Mol. Diagn. 8: 3-15 [Abstract] [Full Text]  
  • Shew, M. L., Fortenberry, J. D., Tu, W., Juliar, B. E., Batteiger, B. E., Qadadri, B., Brown, D. R. (2006). Association of Condom Use, Sexual Behaviors, and Sexually Transmitted Infections With the Duration of Genital Human Papillomavirus Infection Among Adolescent Women. Arch Pediatr Adolesc Med 160: 151-156 [Abstract] [Full Text]  
  • Geraats-Peters, C. W. M., Brouwers, M., Schneeberger, P. M., van der Zanden, A. G. M., Bruisten, S. M., Weers-Pothoff, G., Boel, C. H. E., van den Brule, A. J. C., Harmsen, H. G., Hermans, M. H. A. (2005). Specific and Sensitive Detection of Neisseria gonorrhoeae in Clinical Specimens by Real-Time PCR. J. Clin. Microbiol. 43: 5653-5659 [Abstract] [Full Text]  
  • Lum, G, Freeman, K, Nguyen, N L, Limnios, E A, Tabrizi, S N, Carter, I, Chambers, I W, Whiley, D M, Sloots, T P, Garland, S M, Tapsall, J W (2005). A cluster of culture positive gonococcal infections but with false negative cppB gene based PCR. Sex. Transm. Infect. 81: 400-402 [Abstract] [Full Text]  
  • Boel, C. H. E., van Herk, C. M. C., Berretty, P. J. M., Onland, G. H. W., van den Brule, A. J. C. (2005). Evaluation of Conventional and Real-Time PCR Assays Using Two Targets for Confirmation of Results of the COBAS AMPLICOR Chlamydia trachomatis/Neisseria gonorrhoeae Test for Detection of Neisseria gonorrhoeae in Clinical Samples. J. Clin. Microbiol. 43: 2231-2235 [Abstract] [Full Text]  
  • Luijt, D. S., Bos, P. A. J., van Zwet, A. A., van Voorst Vader, P. C., Schirm, J. (2005). Comparison of COBAS AMPLICOR Neisseria gonorrhoeae PCR, Including Confirmation with N. gonorrhoeae-Specific 16S rRNA PCR, with Traditional Culture. J. Clin. Microbiol. 43: 1445-1447 [Abstract] [Full Text]  
  • Lynn, F., Hobbs, M. M., Zenilman, J. M., Behets, F. M. T. F., Van Damme, K., Rasamindrakotroka, A., Bash, M. C. (2005). Genetic Typing of the Porin Protein of Neisseria gonorrhoeae from Clinical Noncultured Samples for Strain Characterization and Identification of Mixed Gonococcal Infections. J. Clin. Microbiol. 43: 368-375 [Abstract] [Full Text]  
  • Bruisten, S. M., Noordhoek, G. T., van den Brule, A. J. C., Duim, B., Boel, C. H. E., El-Faouzi, K., du Maine, R., Mulder, S., Luijt, D., Schirm, J. (2004). Multicenter Validation of the cppB Gene as a PCR Target for Detection of Neisseria gonorrhoeae. J. Clin. Microbiol. 42: 4332-4334 [Abstract] [Full Text]  
  • Tabrizi, S N, Chen, S, Cohenford, M A, Lentrichia, B B, Coffman, E, Shultz, T, Tapsall, J W, Garland, S M (2004). Evaluation of real time polymerase chain reaction assays for confirmation of Neisseria gonorrhoeae in clinical samples tested positive in the Roche Cobas Amplicor assay. Sex. Transm. Infect. 80: 68-71 [Abstract] [Full Text]  
  • Palmer, H. M., Mallinson, H., Wood, R. L., Herring, A. J. (2003). Evaluation of the Specificities of Five DNA Amplification Methods for the Detection of Neisseria gonorrhoeae. J. Clin. Microbiol. 41: 835-837 [Abstract] [Full Text]  
  • Diemert, D. J., Libman, M. D., Lebel, P. (2002). Confirmation by 16S rRNA PCR of the COBAS AMPLICOR CT/NG Test for Diagnosis of Neisseria gonorrhoeae Infection in a Low-Prevalence Population. J. Clin. Microbiol. 40: 4056-4059 [Abstract] [Full Text]  
  • Gaydos, C. A., Crotchfelt, K. A., Shah, N., Tennant, M., Quinn, T. C., Gaydos, J. C., McKee, K. T. Jr., Rompalo, A. M. (2002). Evaluation of Dry and Wet Transported Intravaginal Swabs in Detection of Chlamydia trachomatis and Neisseria gonorrhoeae Infections in Female Soldiers by PCR. J. Clin. Microbiol. 40: 758-761 [Abstract] [Full Text]  
  • Chaudhry, U, Saluja, D (2002). Detection of Neisseria gonorrhoeae by PCR using orf1 gene as target. Sex. Transm. Infect. 78: 72-72 [Full Text]  
  • Ronpirin, C., Jerse, A. E., Cornelissen, C. N. (2001). Gonococcal Genes Encoding Transferrin-Binding Proteins A and B Are Arranged in a Bicistronic Operon but Are Subject to Differential Expression. Infect. Immun. 69: 6336-6347 [Abstract] [Full Text]  
  • Van Der Pol, B., Martin, D. H., Schachter, J., Quinn, T. C., Gaydos, C. A., Jones, R. B., Crotchfelt, K., Moncada, J., Jungkind, D., Turner, B., Peyton, C., Kelly, J. F., Weiss, J. B., Rosenstraus, M. (2001). Enhancing the Specificity of the COBAS AMPLICOR CT/NG Test for Neisseria gonorrhoeae by Retesting Specimens with Equivocal Results. J. Clin. Microbiol. 39: 3092-3098 [Abstract] [Full Text]  
  • Van Dyck, E., Smet, H., Van Damme, L., Laga, M. (2001). Evaluation of the Roche Neisseria gonorrhoeae 16S rRNA PCR for Confirmation of AMPLICOR PCR-Positive Samples and Comparison of Its Diagnostic Performance According to Storage Conditions and Preparation of Endocervical Specimens. J. Clin. Microbiol. 39: 2280-2282 [Abstract] [Full Text]  
  • Van Dyck, E., Ieven, M., Pattyn, S., Van Damme, L., Laga, M. (2001). Detection of Chlamydia trachomatis and Neisseria gonorrhoeae by Enzyme Immunoassay, Culture, and Three Nucleic Acid Amplification Tests. J. Clin. Microbiol. 39: 1751-1756 [Abstract] [Full Text]  
  • Johnson, S. J., Green, D. H., Reed, D. A., Wood, L. S. (2001). Semiautomation of Nucleic Acid-based Assays for Chlamydia trachomatis and Neisseria gonorrhoeae,. Clin. Chem. 47: 760-763 [Full Text]  
  • van Doornum, G. J. J., Schouls, L. M., Pijl, A., Cairo, I., Buimer, M., Bruisten, S. (2001). Comparison between the LCx Probe System and the COBAS AMPLICOR System for Detection of Chlamydia trachomatis and Neisseria gonorrhoeae Infections in Patients Attending a Clinic for Treatment of Sexually Transmitted Diseases in Amsterdam, The Netherlands. J. Clin. Microbiol. 39: 829-835 [Abstract] [Full Text]  
  • Mukenge-Tshibaka, L., Alary, M., Bernier, F., van Dyck, E., Lowndes, C. M., Guédou, A., Anagonou, S., Joly, J. R. (2000). Diagnostic Performance of the Roche AMPLICOR PCR in Detecting Neisseria gonorrhoeae in Genitourinary Specimens from Female Sex Workers in Cotonou, Benin. J. Clin. Microbiol. 38: 4076-4079 [Abstract] [Full Text]  
  • Martin, D. H., Cammarata, C., Van Der Pol, B., Jones, R. B., Quinn, T. C., Gaydos, C. A., Crotchfelt, K., Schachter, J., Moncada, J., Jungkind, D., Turner, B., Peyton, C. (2000). Multicenter Evaluation of AMPLICOR and Automated COBAS AMPLICOR CT/NG Tests for Neisseria gonorrhoeae. J. Clin. Microbiol. 38: 3544-3549 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»