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Journal of Clinical Microbiology, February 1999, p. 461-463, Vol. 37, No. 2
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

PCR Targeted to the 16S-23S rRNA Gene Intergenic Spacer Region of Clostridium difficile and Construction of a Library Consisting of 116 Different PCR Ribotypes

Simon L. J. Stubbs,1 Jon S. Brazier,1,* Gael L. O'Neill,2 and Brian I. Duerden1

Anaerobe Reference Unit, Department of Medical Microbiology and Public Health Laboratory, University Hospital of Wales, Cardiff,1 and Central Public Health Laboratory, London,2 United Kingdom

Received 8 September 1998/Returned for modification 1 October 1998/Accepted 27 October 1998

A reference library of types of Clostridium difficile has been constructed by PCR ribotyping isolates (n = 2,030) from environmental (n = 89), hospital (n = 1,386), community practitioner (n = 395), veterinary (n = 27), and reference (n = 133) sources. The library consists of 116 distinct types identified on the basis of differences in profiles generated with PCR primers designed to amplify the 16S-23S rRNA gene intergenic spacer region. Isolates from 55% of infections in hospitals in the United Kingdom belonged to one ribotype (type 1), but this type was responsible for only 7.5% of community infections.

* Corresponding author. Mailing address: Anaerobe Reference Unit, Public Health Laboratory, University Hospital of Wales, Heath Park, Cardiff CF4 4XW, United Kingdom. Phone: 01222 742378. Fax: 01222 742161. E-mail: Brazier{at}cf.ac.uk.

Journal of Clinical Microbiology, February 1999, p. 461-463, Vol. 37, No. 2
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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