This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Espinel-Ingroff, A.
Right arrow Articles by Ghannoum, M. A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Espinel-Ingroff, A.
Right arrow Articles by Ghannoum, M. A.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, March 1999, p. 591-595, Vol. 37, No. 3
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Multicenter Comparison of the Sensititre YeastOne Colorimetric Antifungal Panel with the National Committee for Clinical Laboratory Standards M27-A Reference Method for Testing Clinical Isolates of Common and Emerging Candida spp., Cryptococcus spp., and Other Yeasts and Yeast-Like Organisms

A. Espinel-Ingroff,1,* M. Pfaller,2 S. A. Messer,2 C. C. Knapp,3 S. Killian,3 H. A. Norris,4 and M. A. Ghannoum4

Medical College of Virginia of Virginia Commonwealth University, Richmond, Virginia1; University of Iowa College of Medicine, Iowa City, Iowa2; and AccuMed International, Westlake,3 and University Hospital of Cleveland and Case Western Reserve University, Cleveland,4 Ohio

Received 21 September 1998/Returned for modification 21 October 1998/Accepted 11 December 1998

National Committee for Clinical Laboratory Standards (NCCLS) standard guidelines are available for the antifungal susceptibility testing of common Candida spp. and Cryptococcus neoformans, but NCCLS methods may not be the most efficient and convenient procedures for use in the clinical laboratory. MICs of amphotericin B, fluconazole, flucytosine, itraconazole, and ketoconazole were determined by the commercially prepared Sensititre YeastOne Colorimetric Antifungal Panel and by the NCCLS M27-A broth microdilution method for 1,176 clinical isolates of yeasts and yeast-like organisms, including Blastoschizomyces capitatus, Cryptococcus spp., 14 common and emerging species of Candida, Hansenula anomala, Rhodotorula spp., Saccharomyces cerevisiae, Sporobolomyces salmonicolor, and Trichosporon beigelii. Colorimetric MICs of amphotericin B corresponded to the first blue well (no growth), and MICs of the other agents corresponded to the first purple or blue well. Three comparisons of MIC pairs by the two methods were evaluated to obtain percentages of agreement: 24- and 48-h MICs and 24-h colorimetric versus 48-h reference MICs. The best performance of the YeastOne panel was with 24-h MICs (92 to 100%) with the azoles and flucytosine for all the species tested, with the exception of C. albicans (87 to 90%). For amphotericin B, the best agreement between the methods was with 48-h MIC pairs (92 to 99%) for most of the species tested. The exception was for isolates of C. neoformans (76%). These data suggest the potential value of the YeastOne panel for use in the clinical laboratory.

* Corresponding author. Mailing address: Medical College of Virginia of Virginia Commonwealth University, P.O. Box 980049, Richmond, VA 23298-0049. Phone: (804) 828-9711. Fax: (804) 828-3097. E-mail: AVINGROFF{at}HSC.VCU.EDU.

Journal of Clinical Microbiology, March 1999, p. 591-595, Vol. 37, No. 3
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Pfaller, M. A., Boyken, L. B., Hollis, R. J., Kroeger, J., Messer, S. A., Tendolkar, S., Diekema, D. J. (2008). Validation of 24-Hour Fluconazole MIC Readings versus the CLSI 48-Hour Broth Microdilution Reference Method: Results from a Global Candida Antifungal Surveillance Program. J. Clin. Microbiol. 46: 3585-3590 [Abstract] [Full Text]  
  • Ostrosky-Zeichner, L., Rex, J. H., Pfaller, M. A., Diekema, D. J., Alexander, B. D., Andes, D., Brown, S. D., Chaturvedi, V., Ghannoum, M. A., Knapp, C. C., Sheehan, D. J., Walsh, T. J. (2008). Rationale for Reading Fluconazole MICs at 24 Hours Rather than 48 Hours When Testing Candida spp. by the CLSI M27-A2 Standard Method. Antimicrob. Agents Chemother. 52: 4175-4177 [Abstract] [Full Text]  
  • Savini, V., Sozio, F., Catavitello, C., Talia, M., Manna, A., Febbo, F., Balbinot, A., Di Bonaventura, G., Piccolomini, R., Parruti, G., D'Antonio, D. (2008). Femoral Prosthesis Infection by Rhodotorula mucilaginosa. J. Clin. Microbiol. 46: 3544-3545 [Abstract] [Full Text]  
  • Pfaller, M. A., Chaturvedi, V., Diekema, D. J., Ghannoum, M. A., Holliday, N. M., Killian, S. B., Knapp, C. C., Messer, S. A., Miskov, A., Ramani, R. (2008). Clinical Evaluation of the Sensititre YeastOne Colorimetric Antifungal Panel for Antifungal Susceptibility Testing of the Echinocandins Anidulafungin, Caspofungin, and Micafungin. J. Clin. Microbiol. 46: 2155-2159 [Abstract] [Full Text]  
  • Pfaller, M. A., Diekema, D. J., Procop, G. W., Rinaldi, M. G. (2007). Multicenter Comparison of the VITEK 2 Antifungal Susceptibility Test with the CLSI Broth Microdilution Reference Method for Testing Amphotericin B, Flucytosine, and Voriconazole against Candida spp.. J. Clin. Microbiol. 45: 3522-3528 [Abstract] [Full Text]  
  • Alexander, B. D., Byrne, T. C., Smith, K. L., Hanson, K. E., Anstrom, K. J., Perfect, J. R., Reller, L. B. (2007). Comparative Evaluation of Etest and Sensititre YeastOne Panels against the Clinical and Laboratory Standards Institute M27-A2 Reference Broth Microdilution Method for Testing Candida Susceptibility to Seven Antifungal Agents. J. Clin. Microbiol. 45: 698-706 [Abstract] [Full Text]  
  • Pfaller, M. A., Diekema, D. J., Procop, G. W., Rinaldi, M. G. (2007). Multicenter Comparison of the VITEK 2 Yeast Susceptibility Test with the CLSI Broth Microdilution Reference Method for Testing Fluconazole against Candida spp.. J. Clin. Microbiol. 45: 796-802 [Abstract] [Full Text]  
  • Pfaller, M. A., Messer, S. A., Boyken, L., Rice, C., Tendolkar, S., Hollis, R. J., Diekema, D. J. (2007). Use of Fluconazole as a Surrogate Marker To Predict Susceptibility and Resistance to Voriconazole among 13,338 Clinical Isolates of Candida spp. Tested by Clinical and Laboratory Standards Institute-Recommended Broth Microdilution Methods. J. Clin. Microbiol. 45: 70-75 [Abstract] [Full Text]  
  • Espinel-Ingroff, A. (2006). Comparison of Three Commercial Assays and a Modified Disk Diffusion Assay with Two Broth Microdilution Reference Assays for Testing Zygomycetes, Aspergillus spp., Candida spp., and Cryptococcus neoformans with Posaconazole and Amphotericin B. J. Clin. Microbiol. 44: 3616-3622 [Abstract] [Full Text]  
  • Espinel-Ingroff, A., Barchiesi, F., Cuenca-Estrella, M., Fothergill, A., Pfaller, M. A., Rinaldi, M., Rodriguez-Tudela, J. L., Verweij, P. E. (2005). Comparison of Visual 24-Hour and Spectrophotometric 48-Hour MICs to CLSI Reference Microdilution MICs of Fluconazole, Itraconazole, Posaconazole, and Voriconazole for Candida spp.: a Collaborative Study. J. Clin. Microbiol. 43: 4535-4540 [Abstract] [Full Text]  
  • Peter, J., Armstrong, D., Lyman, C. A., Walsh, T. J. (2005). Use of Fluorescent Probes To Determine MICs of Amphotericin B and Caspofungin against Candida spp. and Aspergillus spp.. J. Clin. Microbiol. 43: 3788-3792 [Abstract] [Full Text]  
  • Linares, M. J., Solis, F., Casal, M. (2005). In Vitro Activity of Voriconazole against Prototheca wickerhamii: Comparative Evaluation of Sensititre and NCCLS M27-A2 Methods of Detection. J. Clin. Microbiol. 43: 2520-2522 [Abstract] [Full Text]  
  • van de Sande, W. W. J., Luijendijk, A., Ahmed, A. O. A., Bakker-Woudenberg, I. A. J. M., van Belkum, A. (2005). Testing of the In Vitro Susceptibilities of Madurella mycetomatis to Six Antifungal Agents by Using the Sensititre System in Comparison with a Viability-Based 2,3-Bis(2-Methoxy-4-Nitro-5-Sulfophenyl)-5- [(Phenylamino)Carbonyl]-2H-Tetrazolium Hydroxide (XTT) Assay and a Modified NCCLS Method. Antimicrob. Agents Chemother. 49: 1364-1368 [Abstract] [Full Text]  
  • Canton, E., Peman, J., Gobernado, M., Alvarez, E., Baquero, F., Cisterna, R., Gil, J., Martin-Mazuelos, E., Rubio, C., Sanchez-Sousa, A., Serrano, C. (2005). Sensititre YeastOne Caspofungin Susceptibility Testing of Candida Clinical Isolates: Correlation with Results of NCCLS M27-A2 Multicenter Study. Antimicrob. Agents Chemother. 49: 1604-1607 [Abstract] [Full Text]  
  • Tan, G. L., Peterson, E. M. (2005). CHROMagar Candida Medium for Direct Susceptibility Testing of Yeast from Blood Cultures. J. Clin. Microbiol. 43: 1727-1731 [Abstract] [Full Text]  
  • Gomez-Lopez, A., Mellado, E., Rodriguez-Tudela, J. L., Cuenca-Estrella, M. (2005). Susceptibility profile of 29 clinical isolates of Rhodotorula spp. and literature review. J Antimicrob Chemother 55: 312-316 [Abstract] [Full Text]  
  • Linares, M. J., Charriel, G., Solis, F., Rodriguez, F., Ibarra, A., Casal, M. (2005). Susceptibility of Filamentous Fungi to Voriconazole Tested by Two Microdilution Methods. J. Clin. Microbiol. 43: 250-253 [Abstract] [Full Text]  
  • Pfaller, M. A., Espinel-Ingroff, A., Jones, R. N. (2004). Clinical Evaluation of the Sensititre YeastOne Colorimetric Antifungal Plate for Antifungal Susceptibility Testing of the New Triazoles Voriconazole, Posaconazole, and Ravuconazole. J. Clin. Microbiol. 42: 4577-4580 [Abstract] [Full Text]  
  • Pfaller, M. A., Messer, S. A., Boyken, L., Rice, C., Tendolkar, S., Hollis, R. J., Diekema, D. J. (2004). Cross-Resistance between Fluconazole and Ravuconazole and the Use of Fluconazole as a Surrogate Marker To Predict Susceptibility and Resistance to Ravuconazole among 12,796 Clinical Isolates of Candida spp.. J. Clin. Microbiol. 42: 3137-3141 [Abstract] [Full Text]  
  • Ezra, D., Castillo, U. F., Strobel, G. A., Hess, W. M., Porter, H., Jensen, J. B., Condron, M. A. M., Teplow, D. B., Sears, J., Maranta, M., Hunter, M., Weber, B., Yaver, D. (2004). Coronamycins, peptide antibiotics produced by a verticillate Streptomyces sp. (MSU-2110) endophytic on Monstera sp.. Microbiology 150: 785-793 [Abstract] [Full Text]  
  • Espinel-Ingroff, A., Pfaller, M., Messer, S. A., Knapp, C. C., Holliday, N., Killian, S. B. (2004). Multicenter Comparison of the Sensititre YeastOne Colorimetric Antifungal Panel with the NCCLS M27-A2 Reference Method for Testing New Antifungal Agents against Clinical Isolates of Candida spp.. J. Clin. Microbiol. 42: 718-721 [Abstract] [Full Text]  
  • Linares, M. J., Charriel, G., Solis, F., Casal, M. (2004). Comparison of Two Microdilution Methods for Testing Susceptibility of Candida spp. to Voriconazole. J. Clin. Microbiol. 42: 899-902 [Abstract] [Full Text]  
  • Zaas, A. K., Boyce, M., Schell, W., Lodge, B. A., Miller, J. L., Perfect, J. R. (2003). Risk of Fungemia Due to Rhodotorula and Antifungal Susceptibility Testing of Rhodotorula Isolates. J. Clin. Microbiol. 41: 5233-5235 [Abstract] [Full Text]  
  • Martin-Mazuelos, E., Peman, J., Valverde, A., Chaves, M., Serrano, M. C., Canton, E. (2003). Comparison of the Sensititre YeastOne colorimetric antifungal panel and Etest with the NCCLS M38-A method to determine the activity of amphotericin B and itraconazole against clinical isolates of Aspergillus spp.. J Antimicrob Chemother 52: 365-370 [Abstract] [Full Text]  
  • Ramani, R., Chaturvedi, V. (2003). Proficiency Testing Program for Clinical Laboratories Performing Antifungal Susceptibility Testing of Pathogenic Yeast Species. J. Clin. Microbiol. 41: 1143-1146 [Abstract] [Full Text]  
  • Moore, C. B., Walls, C. M., Denning, D. W. (2003). Comparison of three methods for in vitro susceptibility testing of Candida species with flucytosine. J Antimicrob Chemother 51: 297-304 [Abstract] [Full Text]  
  • Meletiadis, J., Mouton, J. W., Meis, J. F. G. M., Bouman, B. A., Verweij, P. E. (2002). Comparison of the Etest and the Sensititre Colorimetric Methods with the NCCLS Proposed Standard for Antifungal Susceptibility Testing of Aspergillus Species. J. Clin. Microbiol. 40: 2876-2885 [Abstract] [Full Text]  
  • Pujol, I., Capilla, J., Fernandez-Torres, B., Ortoneda, M., Guarro, J. (2002). Use of the Sensititre Colorimetric Microdilution Panel for Antifungal Susceptibility Testing of Dermatophytes. J. Clin. Microbiol. 40: 2618-2621 [Abstract] [Full Text]  
  • Chryssanthou, E. (2001). Trends in Antifungal Susceptibility among Swedish Candida Species Bloodstream Isolates from 1994 to 1998: Comparison of the E-test and the Sensititre YeastOne Colorimetric Antifungal Panel with the NCCLS M27-A Reference Method. J. Clin. Microbiol. 39: 4181-4183 [Abstract] [Full Text]  
  • Rex, J. H., Pfaller, M. A., Walsh, T. J., Chaturvedi, V., Espinel-Ingroff, A., Ghannoum, M. A., Gosey, L. L., Odds, F. C., Rinaldi, M. G., Sheehan, D. J., Warnock, D. W. (2001). Antifungal Susceptibility Testing: Practical Aspects and Current Challenges. Clin. Microbiol. Rev. 14: 643-658 [Abstract] [Full Text]  
  • Nakamura, Y., Kano, R., Murai, T., Watanabe, S., Hasegawa, A. (2000). Susceptibility Testing of Malassezia Species Using the Urea Broth Microdilution Method. Antimicrob. Agents Chemother. 44: 2185-2186 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»