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Journal of Clinical Microbiology, March 1999, p. 715-720, Vol. 37, No. 3
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Genetic Multilocus Studies of Different Strains of Cryptococcus neoformans: Taxonomy and Genetic Structure

S. Bertout,1 F. Renaud,2 D. Swinne,3 M. Mallié,1 and J.-M. Bastide1,*

Laboratoire d'Immunologie Parasitologie MENRT UPRES EA 2413, Université Montpellier I, Faculté de Pharmacie, 34060 Montpellier Cédex 2,1 and Centre d'Étude sur le Polymorphisme des Micro-Organismes, CEPM/UMR CNRS-ORSTOM 9926, ORSTOM, 34032, Montpellier Cédex 1,2 France, and Institut de Médecine Tropicale Prince Léopold, 2000 Antwerp, Belgium3

Received 18 August 1998/Returned for modification 15 October 1998/Accepted 30 November 1998

The genotypes of 107 strains of Cryptococcus isolated from the environment or from patients from various geographical areas were determined by multilocus enzyme electrophoresis (MLEE). We analyzed the relationships between genotype structure and serotype and between genotype structure and strain origin. Twelve of the 14 enzyme-encoding loci studied were polymorphic, giving rise to 48 electrophoretic types. The genotypes of C. neoformans and C. laurentii were very similar. MLEE could not distinguish between these two pathogenic species. A correlation between the genetic multilocus structure and the origin of the sample (from the environment or patients) existed. A second analysis detected a correlation between genotype distribution and serotype. The second analysis considered three serotype groups (B, C, and A plus D plus A/D), proving that serotypes A, D, and A/D are closely related. MLEE is a useful epidemiological tool for improving our understanding of the biology of this fungus.

* Corresponding author. Present address: Laboratoire d'Immunologie et Parasitologie, Faculté de Pharmacie, 15, Av. Charles Flahault, 34060 Montpellier Cédex 2, France. Phone: 33.4.67.63.52.02. Fax: 33.4.67.41.16.17. E-mail: Jean-Marie.Bastide{at}pharma.univ-montp1.fr.

Journal of Clinical Microbiology, March 1999, p. 715-720, Vol. 37, No. 3
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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