This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Felten, A.
Right arrow Articles by Philippon, A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Felten, A.
Right arrow Articles by Philippon, A.
Right arrowPubmed/NCBI databases
*Compound via MeSH
*Substance via MeSH
Medline Plus Health Information
*Antibiotics
Hazardous Substances DB
*HYDROGEN PEROXIDE

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, March 1999, p. 729-733, Vol. 37, No. 3
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Lactobacillus Species Identification, H2O2 Production, and Antibiotic Resistance and Correlation with Human Clinical Status

Annie Felten,1,* Claude Barreau,2 Chantal Bizet,2 Philippe Henri Lagrange,1 and Alain Philippon3

Service de Bactériologie-Virologie-Hygiène, Hôpital St-Louis, 75475 Paris Cedex 10,1 Collection de l'Institut Pasteur, 75724 Paris Cedex 15,2 and Service de Bactériologie-Virologie, Hôpital Cochin, 75679 Paris Cedex 14,3 France

Received 15 July 1998/Returned for modification 31 August 1998/Accepted 5 November 1998

Lactobacilli recovered from the blood, cerebrospinal fluid, respiratory tract, and gut of 20 hospitalized immunocompromised septic patients were analyzed. Biochemical carbohydrate fermentation and total soluble cell protein profiles were used to identify the species. Hydrogen peroxide production was measured. Susceptibility to 19 antibiotics was tested by a diffusion method, and the MICs of benzylpenicillin, amoxicillin, imipenem, erythromycin, vancomycin, gentamicin, and levofloxacin were determined. A small number of species produced H2O2, and antibiotic susceptibilities were species related. Eighteen (90%) of the isolates were L. rhamnosus, one was L. paracasei subsp. paracasei, and one was L. crispatus. L. rhamnosus, L. paracasei subsp. paracasei isolates, and the type strains were neither H2O2 producers nor vancomycin susceptible (MICs, >= 256 µg/ml). L. crispatus, as well as most of the type strains of lactobacilli which belong to the L. acidophilus group, was an H2O2 producer and vancomycin susceptible (MICs, <4 µg/ml).

* Corresponding author. Mailing address: Service de Microbiologie, Hôpital Saint-Louis, 1 avenue Vellefaux, 75475 Paris Cedex 10, France. Phone: 33 1 42 49 93 48. Fax: 33 1 42 49 92 00.

Journal of Clinical Microbiology, March 1999, p. 729-733, Vol. 37, No. 3
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Spurbeck, R. R., Arvidson, C. G. (2008). Inhibition of Neisseria gonorrhoeae Epithelial Cell Interactions by Vaginal Lactobacillus Species. Infect. Immun. 76: 3124-3130 [Abstract] [Full Text]  
  • Klare, I., Konstabel, C., Werner, G., Huys, G., Vankerckhoven, V., Kahlmeter, G., Hildebrandt, B., Muller-Bertling, S., Witte, W., Goossens, H. (2007). Antimicrobial susceptibilities of Lactobacillus, Pediococcus and Lactococcus human isolates and cultures intended for probiotic or nutritional use. J Antimicrob Chemother 59: 900-912 [Abstract] [Full Text]  
  • Klare, I., Konstabel, C., Muller-Bertling, S., Reissbrodt, R., Huys, G., Vancanneyt, M., Swings, J., Goossens, H., Witte, W. (2005). Evaluation of New Broth Media for Microdilution Antibiotic Susceptibility Testing of Lactobacilli, Pediococci, Lactococci, and Bifidobacteria. Appl. Environ. Microbiol. 71: 8982-8986 [Abstract] [Full Text]  
  • Christensen, J. E., Reynolds, C. E., Shukla, S. K., Reed, K. D. (2004). Rapid Molecular Diagnosis of Lactobacillus Bacteremia by Terminal Restriction Fragment Length Polymorphism Analysis of the 16S rRNA Gene. Clin Med Res 2: 37-45 [Abstract] [Full Text]  
  • Collier, C. T., van der Klis, J. D., Deplancke, B., Anderson, D. B., Gaskins, H. R. (2003). Effects of Tylosin on Bacterial Mucolysis, Clostridium perfringens Colonization, and Intestinal Barrier Function in a Chick Model of Necrotic Enteritis. Antimicrob. Agents Chemother. 47: 3311-3317 [Abstract] [Full Text]  
  • Rabe, L. K., Hillier, S. L. (2003). Optimization of Media for Detection of Hydrogen Peroxide Production by Lactobacillus Species. J. Clin. Microbiol. 41: 3260-3264 [Abstract] [Full Text]  
  • ANNUK, H., HYNES, S. O., HIRMO, S., MIKELSAAR, M., WADSTROM, T. (2001). Characterisation and differentiation of lactobacilli by lectin typing. J Med Microbiol 50: 1069-1074 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»