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Journal of Clinical Microbiology, March 1999, p. 772-774, Vol. 37, No. 3
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Comparison of Five PCR Methods for Detection of Helicobacter pylori DNA in Gastric Tissues

Jang-Jih Lu,1,* Cherng-Lih Perng,1 Rong-Yaun Shyu,2 Chi-Hsiang Chen,1 Qinyuan Lou,3 Sonny K. F. Chong,3 and Chao-Hung Lee4

Division of Clinical Pathology, Department of Pathology,1 and Department of Internal Medicine,2 Tri-Service General Hospital and National Defense Medical Center, Taipei, Taiwan; and Departments of Pediatric Gastroenterology and Nutrition3 and Pathology and Laboratory Medicine,4 Indiana University School of Medicine, Indianapolis, Indiana 46202

Received 24 August 1998/Returned for modification 2 November 1998/Accepted 3 December 1998

Five different PCR methods for the detection of Helicobacter pylori were evaluated. The results of this study indicate that of the five PCR methods examined, the ureC (glmM) gene PCR is the most sensitive and specific for the detection of H. pylori in gastric biopsy specimens.

* Corresponding author. Mailing address: Molecular Diagnostics Laboratory, Division of Clinical Pathology, Department of Pathology, Tri-Service General Hospital, No. 8, Section 3, Ting-Chow Rd., Taipei, Taiwan, R.O.C. Phone: 886-2-2368-0235. Fax: 886-2-2368-0235. E-mail: JJL{at}NDMC1.NDMCTSGH.EDU.TW.

Journal of Clinical Microbiology, March 1999, p. 772-774, Vol. 37, No. 3
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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