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Journal of Clinical Microbiology, March 1999, p. 804-806, Vol. 37, No. 3
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Application of Dried Blood Spot Specimens for Serologic Subtyping of Human Immunodeficiency Virus Type 1 in Thailand

P. Chanbancherd,1 A. E. Brown,2 R. Trichavaroj,2 P. Tienamporn,1 P. Puthakird,1 N. Limpairojn,1 T. C. VanCott,3 and M. S. de Souza2,4,*

Army Institute of Pathology,1 Armed Forces Research Institute for Medical Sciences,2 and Henry M. Jackson Foundation for the Advancement of Military Medicine,4 Bangkok, Thailand, and Henry M. Jackson Foundation for the Advancement of Military Medicine, Rockville, Maryland3

Received 20 May 1998/Returned for modification 5 October 1998/Accepted 25 November 1998

Dried blood spot (DBS) specimens were assessed as an alternative to plasma for human immunodeficiency virus type 1 (HIV-1) serotyping by V3 loop peptide enzyme immunoassay. Nested PCR capable of distinguishing HIV-1 subtypes B and E was used as the reference standard. Ninety-two percent of DBS samples were typeable as either HIV-1 subtype B or E. Serotype results with DBS and plasma were identical for 254 of 257 specimens. A simple DBS collection method provides a convenient alternative for conducting HIV-1 serotype surveillance while retaining sensitivity and specificity.

* Corresponding author. Mailing address: Henry M. Jackson Foundation for the Advancement of Military Medicine, AFRIMS, 315/6 Rajvithi Road, Bangkok 10400, Thailand. Phone: 66-2-644-4888, ext. 1504. Fax: 66-2-644-4824. E-mail: mdesouza{at}hiv.hjf.org.

Journal of Clinical Microbiology, March 1999, p. 804-806, Vol. 37, No. 3
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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