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Journal of Clinical Microbiology, March 1999, p. 828-829, Vol. 37, No. 3
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Unexpected Cross-Reaction with Fusobacterium necrophorum in a PCR for Detection of Mycoplasmas

Jørgen Skov Jensen,1,* Brita Bruun,2 and Bente Gahrn-Hansen3

Mycoplasma Laboratory, Neisseria Department,1 and Department of Clinical Microbiology,2 Statens Serum Institut, Copenhagen, and Department of Clinical Microbiology, Odense University Hospital, Odense,3 Denmark

Received 1 July 1998/Returned for modification 30 September 1998/Accepted 30 November 1998

Cross-reactions with Fusobacterium necrophorum were found in a PCR designed for detection of a wide range of mycoplasma species. Twenty-five strains of Fusobacterium were examined; all 14 F. necrophorum strains reacted positively, whereas all 7 Fusobacterium nucleatum strains reacted negatively. Two strains that were not F. necrophorum yielded variable results.

* Corresponding author. Mailing address: Mycoplasma Laboratory, Neisseria Department, Statens Serum Institut, Artillerivej 5, DK-2300 Copenhagen S, Denmark. Phone: 45 3268 3636. Fax: 45 3268 3152. E-mail: jsj{at}ssi.dk.

Journal of Clinical Microbiology, March 1999, p. 828-829, Vol. 37, No. 3
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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  • Riordan, T, Wilson, M (2004). Lemierre's syndrome: more than a historical curiosa. Postgrad. Med. J. 80: 328-334 [Abstract] [Full Text]  
  • Kong, F., James, G., Gordon, S., Zelynski, A., Gilbert, G. L. (2001). Species-Specific PCR for Identification of Common Contaminant Mollicutes in Cell Culture. Appl. Environ. Microbiol. 67: 3195-3200 [Abstract] [Full Text]  


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