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Journal of Clinical Microbiology, March 1999, p. 835-837, Vol. 37, No. 3
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Justification for Use of a Single Trichrome Stain as the Sole Means for Routine Detection of Intestinal Parasites in Concentrated Stool Specimens

James A. Kellogg* and Carol J. Elder

Clinical Microbiology Laboratory, York Hospital, York, Pennsylvania

Received 23 July 1998/Returned for modification 21 October 1998/Accepted 18 November 1998

Of 12,321 stool samples analyzed over a 6-year interval, 870 (7.1%) were positive for a total of 1,019 parasites, of which 1,011 (99.2%) were found in trichrome-stained smears of unconcentrated specimens while only 479 (47.0%) were detected in iodine-stained smears of concentrated samples. Stool specimens were next analyzed by trichrome staining of both unconcentrated and concentrated specimens preserved in either mercury-polyvinyl alcohol (PVA) or cupric PVA. Of 2,198 specimens, 171 (7.8%) were positive for a total of 208 parasites, 192 (92.3%) and 204 (98.1%) of which were found in the unconcentrated and concentrated specimens, respectively (P < 0.05). In our patient population, examination of a single trichrome-stained smear of a concentrated stool specimen is a cost-effective alternative to routinely analyzing both concentrated and unconcentrated specimens for parasites.

* Corresponding author. Mailing address: Clinical Microbiology Laboratory, York Hospital, 1001 S. George St., York, PA 17405. Phone: (717) 851-2393. Fax: (717) 851-2707. E-mail: jkellogg{at}yorkhospital.edu.

Journal of Clinical Microbiology, March 1999, p. 835-837, Vol. 37, No. 3
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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