This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Manavathu, E. K.
Right arrow Articles by Chandrasekar, P. H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Manavathu, E. K.
Right arrow Articles by Chandrasekar, P. H.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, March 1999, p. 858-861, Vol. 37, No. 3
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Comparative Study of Susceptibilities of Germinated and Ungerminated Conidia of Aspergillus fumigatus to Various Antifungal Agents

Elias K. Manavathu,* Jessica Cutright, and Pranatharthi H. Chandrasekar

Division of Infectious Diseases, Department of Medicine, Wayne State University School of Medicine, Detroit, Michigan 48201

Received 3 August 1998/Returned for modification 22 September 1998/Accepted 1 December 1998

Conidia are used as inocula for the in vitro susceptibility testing of Aspergillus fumigatus. Since the MIC is defined on the basis of visible mycelial growth, conidia should germinate and produce sporelings (germinated conidia) for monitoring of the growth inhibition and fungicidal activity of a drug. If a compound is capable of inhibiting germination of conidia while affecting or not affecting the growth of the organism, the MIC obtained will be the concentration of the drug required for the inhibition of conidial germination but not necessarily that required for inhibition of the growth of the organism. We investigated the susceptibility of germinated and ungerminated conidia to amphotericin B, itraconazole, voriconazole, and SCH56592. The MICs of various antifungal agents for germinated conidia were almost identical to those obtained for ungerminated conidia. In addition, both the germinated and ungerminated conidia were killed with almost equal efficiency by all of the compounds tested when exposed to the drugs for 24 h. These results suggest that either germinated or ungerminated conidia could be used as inocula for in vitro susceptibility studies of A. fumigatus with identical results.

* Corresponding author. Mailing address: Department of Medicine, 427 Lande Building, 550 E. Canfield, Detroit, MI 48201. Phone: (313) 577-1931. Fax: (313) 993-0302. E-mail: aa1388{at}wayne.edu.

Journal of Clinical Microbiology, March 1999, p. 858-861, Vol. 37, No. 3
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Serbina, N. V., Cherny, M., Shi, C., Bleau, S. A., Collins, N. H., Young, J. W., Pamer, E. G. (2009). Distinct Responses of Human Monocyte Subsets to Aspergillus fumigatus Conidia. J. Immunol. 183: 2678-2687 [Abstract] [Full Text]  
  • Bellanger, A.-P., Millon, L., Khoufache, K., Rivollet, D., Bieche, I., Laurendeau, I., Vidaud, M., Botterel, F., Bretagne, S. (2009). Aspergillus fumigatus germ tube growth and not conidia ingestion induces expression of inflammatory mediator genes in the human lung epithelial cell line A549. J Med Microbiol 58: 174-179 [Abstract] [Full Text]  
  • Richards, T. S., Oliver, B. G., White, T. C. (2008). Micafungin activity against Candida albicans with diverse azole resistance phenotypes. J Antimicrob Chemother 62: 349-355 [Abstract] [Full Text]  
  • Chaturvedi, A. K., Kavishwar, A., Shiva Keshava, G. B., Shukla, P. K. (2005). Monoclonal Immunoglobulin G1 Directed against Aspergillus fumigatus Cell Wall Glycoprotein Protects against Experimental Murine Aspergillosis. CVI 12: 1063-1068 [Abstract] [Full Text]  
  • Krishnan, S., Manavathu, E. K., Chandrasekar, P. H. (2005). A comparative study of fungicidal activities of voriconazole and amphotericin B against hyphae of Aspergillus fumigatus. J Antimicrob Chemother 55: 914-920 [Abstract] [Full Text]  
  • Araujo, R., Rodrigues, A. G. (2004). Variability of Germinative Potential among Pathogenic Species of Aspergillus. J. Clin. Microbiol. 42: 4335-4337 [Abstract] [Full Text]  
  • Araujo, R., Rodrigues, A. G., Pina-Vaz, C. (2004). A fast, practical and reproducible procedure for the standardization of the cell density of an Aspergillus suspension. J Med Microbiol 53: 783-786 [Abstract] [Full Text]  
  • Fernandez-Torres, B., Inza, I., Guarro, J. (2003). Comparison of In Vitro Antifungal Susceptibilities of Conidia and Hyphae of Dermatophytes with Thick-Wall Macroconidia. Antimicrob. Agents Chemother. 47: 3371-3372 [Full Text]  
  • Favre, B., Hofbauer, B., Hildering, K.-S., Ryder, N. S. (2003). Comparison of In Vitro Activities of 17 Antifungal Drugs against a Panel of 20 Dermatophytes by Using a Microdilution Assay. J. Clin. Microbiol. 41: 4817-4819 [Abstract] [Full Text]  
  • Espinel-Ingroff, A., Fothergill, A., Peter, J., Rinaldi, M. G., Walsh, T. J. (2002). Testing Conditions for Determination of Minimum Fungicidal Concentrations of New and Established Antifungal Agents for Aspergillus spp.: NCCLS Collaborative Study. J. Clin. Microbiol. 40: 3204-3208 [Abstract] [Full Text]  
  • Hammer, K. A., Carson, C. F., Riley, T. V. (2002). In vitro activity of Melaleuca alternifolia (tea tree) oil against dermatophytes and other filamentous fungi. J Antimicrob Chemother 50: 195-199 [Abstract] [Full Text]  
  • Vitale, R. G., Mouton, J. W., Afeltra, J., Meis, J. F. G. M., Verweij, P. E. (2002). Method for Measuring Postantifungal Effect in Aspergillus Species. Antimicrob. Agents Chemother. 46: 1960-1965 [Abstract] [Full Text]  
  • Gonzalez, G. M., Tijerina, R., Sutton, D. A., Graybill, J. R., Rinaldi, M. G. (2002). In Vitro Activities of Free and Lipid Formulations of Amphotericin B and Nystatin against Clinical Isolates of Coccidioides immitis at Various Saprobic Stages. Antimicrob. Agents Chemother. 46: 1583-1585 [Abstract] [Full Text]  
  • Cenci, E., Mencacci, A., Spreca, A., Montagnoli, C., Bacci, A., Perruccio, K., Velardi, A., Magliani, W., Conti, S., Polonelli, L., Romani, L. (2002). Protection of Killer Antiidiotypic Antibodies against Early Invasive Aspergillosis in a Murine Model of Allogeneic T-Cell-Depleted Bone Marrow Transplantation. Infect. Immun. 70: 2375-2382 [Abstract] [Full Text]  
  • Rex, J. H., Pfaller, M. A., Walsh, T. J., Chaturvedi, V., Espinel-Ingroff, A., Ghannoum, M. A., Gosey, L. L., Odds, F. C., Rinaldi, M. G., Sheehan, D. J., Warnock, D. W. (2001). Antifungal Susceptibility Testing: Practical Aspects and Current Challenges. Clin. Microbiol. Rev. 14: 643-658 [Abstract] [Full Text]  
  • Fernandez-Torres, B., Carrillo, A. J., Martin, E., Del Palacio, A., Moore, M. K., Valverde, A., Serrano, M., Guarro, J. (2001). In Vitro Activities of 10 Antifungal Drugs against 508 Dermatophyte Strains. Antimicrob. Agents Chemother. 45: 2524-2528 [Abstract] [Full Text]  
  • Pujol, I., Fernandez-Ballart, J., Guarro, J. (2001). Effect of inoculum form on in vitro antifungal susceptibilities of Aspergillus spp.. J Antimicrob Chemother 47: 715-718 [Abstract] [Full Text]  
  • Espinel-Ingroff, A. (2001). In Vitro Fungicidal Activities of Voriconazole, Itraconazole, and Amphotericin B against Opportunistic Moniliaceous and Dematiaceous Fungi. J. Clin. Microbiol. 39: 954-958 [Abstract] [Full Text]  
  • Espinel-Ingroff, A. (2001). Germinated and Nongerminated Conidial Suspensions for Testing of Susceptibilities of Aspergillus spp. to Amphotericin B, Itraconazole, Posaconazole, Ravuconazole, and Voriconazole. Antimicrob. Agents Chemother. 45: 605-607 [Abstract] [Full Text]  
  • Lass-Flörl, C., Nagl, M., Speth, C., Ulmer, H., Dierich, M. P., Würzner, R. (2001). Studies of In Vitro Activities of Voriconazole and Itraconazole against Aspergillus Hyphae Using Viability Staining. Antimicrob. Agents Chemother. 45: 124-128 [Abstract] [Full Text]  
  • Perea, S., Fothergill, A. W., Sutton, D. A., Rinaldi, M. G. (2001). Comparison of In Vitro Activities of Voriconazole and Five Established Antifungal Agents against Different Species of Dermatophytes Using a Broth Macrodilution Method. J. Clin. Microbiol. 39: 385-388 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»