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Journal of Clinical Microbiology, April 1999, p. 1004-1007, Vol. 37, No. 4
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Comparison of Isolation Media for Recovery of Burkholderia cepacia Complex from Respiratory Secretions of Patients with Cystic Fibrosis

Deborah Henry,1 Maureen Campbell,1 Colleen McGimpsey,2 Alison Clarke,2 Laurie Louden,3 Jane L. Burns,3,4 Martha H. Roe,5 Peter Vandamme,6 and David Speert1,*

Division of Infectious and Immunological Diseases, Department of Pediatrics, University of British Columbia,1 and Department of Microbiology, Saint Paul's Hospital,2 Vancouver, British Columbia, Canada; Department of Pediatrics, Children's Hospital and Regional Medical Center,3 and Division of Infectious Disease, University of Washington School of Medicine,4 Seattle, Washington; Department of Pathology, The Children's Hospital, Denver, Colorado5; and Laboratory of Microbiology, Universiteit Gent, Ghent, Belgium6

Received 5 October 1998/Returned for modification 23 November 1998/Accepted 13 January 1999

Burkholderia cepacia selective agar (BCSA) has previously been devised for isolation of B. cepacia from respiratory secretions of patients with cystic fibrosis and tested under research laboratory conditions. Here we describe a study in which BCSA, oxidation-fermentation polymyxin bacitracin lactose agar (OFPBL), and Pseudomonas cepacia agar (PCA) were compared in routine culture procedures for the ability to grow B. cepacia and inhibit other organisms. Three hundred twenty-eight specimens from 209 patients at two pediatric centers and 328 specimens from 109 adults were tested. Plates were inoculated, incubated, and read for quality and quantity of growth at 24, 48, and 72 h. Five (1.5%) specimens from 4 (1.9%) children and 75 (22.9%) specimens from 16 (14.7%) adults grew B. cepacia complex. At 24, 48, and 72 h, BCSA achieved 43, 93, and 100% detection, respectively; OFPBL achieved 26, 84, and 96%, respectively; and PCA achieved 33, 74, and 84% detection, respectively. Quality was assessed as pinpoint or good growth. At 24 h, most cultures growing B. cepacia complex had pinpoint colonies. By 48 and 72 h, 48 and 69% of B. cepacia complex cultures, respectively, had good growth on BCSA, while on OFPBL 19 and 30%, respectively, had good growth and on PCA 11 and 18%, respectively, had good growth. BCSA was superior to OFPBL and PCA in suppressing organisms other than B. cepacia complex; 40 non-B. cepacia complex organisms were isolated from BCSA, 263 were isolated from OFPBL, and 116 were isolated from PCA. We conclude that BCSA is superior to OFPBL and PCA in its ability to support the growth of B. cepacia complex and to suppress other respiratory organisms.

* Corresponding author. Mailing address: BC Research Institute of Women's and Children's Health, Room 375, 950 West 28th Ave., Vancouver, BC V5Z 4H4, Canada. Phone: (604) 875-2438. Fax: (604) 875-2226. E-mail: speert{at}unixg.ubc.ca.

Journal of Clinical Microbiology, April 1999, p. 1004-1007, Vol. 37, No. 4
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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