This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Whittington, R. J.
Right arrow Articles by Fraser, C. A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Whittington, R. J.
Right arrow Articles by Fraser, C. A.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, April 1999, p. 1077-1083, Vol. 37, No. 4
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Evaluation of Modified BACTEC 12B Radiometric Medium and Solid Media for Culture of Mycobacterium avium subsp. paratuberculosis from Sheep

R. J. Whittington,1,* I. Marsh,1 S. McAllister,1 M. J. Turner,1 D. J. Marshall,2 and C. A. Fraser2

NSW Agriculture, Elizabeth Macarthur Agricultural Institute, Camden, New South Wales 2570,1 and Orange Agricultural Institute, Orange, New South Wales 2500,2 Australia

Received 26 August 1998/Returned for modification 11 December 1998/Accepted 19 January 1999

Definitive diagnosis of Johne's disease in ruminants depends on confirming the presence of the causative bacterium, Mycobacterium avium subsp. paratuberculosis, in tissues of the host. This is readily achieved in most ruminant species by culture. However, culture of clinical specimens from sheep in many countries has been unrewarding. Such a culture from sheep was achieved recently in Australia by using a radiometric culture medium. The aims of the present study were to evaluate the culture of M. avium subsp. paratuberculosis from sheep by using modified BACTEC 12B radiometric medium, to determine the sensitivity of culture in relation to histopathology, and to evaluate a range of solid media. Culture of M. avium subsp. paratuberculosis from sheep with Johne's disease is a sensitive method of diagnosis: intestinal tissues from all 43 animals with multibacillary disease and all 22 animals with paucibacillary disease were culture positive, while 98% of feces from 53 animals with multibacillary disease and 48% of feces from 31 animals with paucibacillary disease were culture positive. Of sheep without histological evidence of Johne's disease from infected flocks, intestinal tissue from 32% of 41 were culture positive, while feces from 17% of 41 were culture positive. Consequently, culture is recommended as the "gold standard" test for detection of ovine Johne's disease. Of the wide range of solid media that were evaluated, only modified Middlebrook 7H10 and 7H11 agars, which were very similar in composition to modified BACTEC 12B medium, yielded growth of ovine strains of M. avium subsp. paratuberculosis. The sensitivity of detection of M. avium subsp. paratuberculosis on solid media was slightly lower than that in modified BACTEC 12B radiometric medium. Both egg yolk and mycobactin J were essential additives for growth of ovine strains of M. avium subsp. paratuberculosis in both liquid and solid media.

* Corresponding author. Mailing address: NSW Agriculture, Elizabeth Macarthur Agricultural Institute, PMB 8, Camden NSW 2570, Australia. Phone: 61 46406343. Fax: 61 46406384. E-mail: Richard.Whittington{at}agric.nsw.gov.au.

Journal of Clinical Microbiology, April 1999, p. 1077-1083, Vol. 37, No. 4
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Dhand, N. K., Toribio, J.-A. L. M. L., Whittington, R. J. (2009). Adsorption of Mycobacterium avium subsp. paratuberculosis to Soil Particles. Appl. Environ. Microbiol. 75: 5581-5585 [Abstract] [Full Text]  
  • Begg, D. J., de Silva, K., Bosward, K., Di Fiore, L., Taylor, D. L., Jungersen, G., Whittington, R. J. (2009). Enzyme-linked immunospot: an alternative method for the detection of interferon gamma in Johne's disease. jvdi 21: 187-196 [Abstract] [Full Text]  
  • Whittington, R. J. (2009). Factors Affecting Isolation and Identification of Mycobacterium avium subsp. paratuberculosis from Fecal and Tissue Samples in a Liquid Culture System. J. Clin. Microbiol. 47: 614-622 [Abstract] [Full Text]  
  • Turenne, C. Y., Wallace, R. Jr., Behr, M. A. (2007). Mycobacterium avium in the Postgenomic Era. Clin. Microbiol. Rev. 20: 205-229 [Abstract] [Full Text]  
  • Radosevich, T. J., Reinhardt, T. A., Lippolis, J. D., Bannantine, J. P., Stabel, J. R. (2007). Proteome and Differential Expression Analysis of Membrane and Cytosolic Proteins from Mycobacterium avium subsp. paratuberculosis Strains K-10 and 187. J. Bacteriol. 189: 1109-1117 [Abstract] [Full Text]  
  • Rajeev, S., Shulaw, W., Berghaus, R., Zhang, Y., Byrum, B. (2006). A testing scheme for the detection of Mycobacterium avium subsp. Paratuberculosis in bovine feces utilizing the ESP Para-JEM liquid culture system. jvdi 18: 529-535 [Abstract] [Full Text]  
  • de Juan, L., Alvarez, J., Romero, B., Bezos, J., Castellanos, E., Aranaz, A., Mateos, A., Dominguez, L. (2006). Comparison of Four Different Culture Media for Isolation and Growth of Type II and Type I/III Mycobacterium avium subsp. paratuberculosis Strains Isolated from Cattle and Goats. Appl. Environ. Microbiol. 72: 5927-5932 [Abstract] [Full Text]  
  • Berger, S., Hinz, D., Bannantine, J. P., Griffin, J. F. T. (2006). Isolation of High-Affinity Single-Chain Antibodies against Mycobacterium avium subsp. paratuberculosis Surface Proteins from Sheep with Johne's Disease.. CVI 13: 1022-1029 [Abstract] [Full Text]  
  • Begg, D. J., O'Brien, R., Mackintosh, C. G., Griffin, J. F. T. (2005). Experimental Infection Model for Johne's Disease in Sheep. Infect. Immun. 73: 5603-5611 [Abstract] [Full Text]  
  • Whittington, R. J., Marshall, D. J., Nicholls, P. J., Marsh, I. B., Reddacliff, L. A. (2004). Survival and Dormancy of Mycobacterium avium subsp. paratuberculosis in the Environment. Appl. Environ. Microbiol. 70: 2989-3004 [Abstract] [Full Text]  
  • Parrish, N. M., Ko, C. G., Dick, J. D., Jones, P. B., Ellingson, J. L.E. (2004). Growth, Congo Red Agar Colony Morphotypes and Antibiotic Susceptibility Testing of Mycobacterium avium subspecies paratuberculosis. Clin Med Res 2: 107-114 [Abstract] [Full Text]  
  • Reddacliff, L. A., Nicholls, P. J., Vadali, A., Whittington, R. J. (2003). Use of Growth Indices from Radiometric Culture for Quantification of Sheep Strains of Mycobacterium avium subsp. paratuberculosis. Appl. Environ. Microbiol. 69: 3510-3516 [Abstract] [Full Text]  
  • Thornton, C. G., MacLellan, K. M., Stabel, J. R., Carothers, C., Whitlock, R. H., Passen, S. (2002). Application of the C18-Carboxypropylbetaine Specimen Processing Method to Recovery of Mycobacterium avium subsp. paratuberculosis from Ruminant Tissue Specimens. J. Clin. Microbiol. 40: 1783-1790 [Abstract] [Full Text]  
  • Collins, M. T., Lisby, G., Moser, C., Chicks, D., Christensen, S., Reichelderfer, M., Høiby, N., Harms, B. A., Thomsen, O. O., Skibsted, U., Binder, V. (2000). Results of Multiple Diagnostic Tests for Mycobacterium avium subsp. paratuberculosis in Patients with Inflammatory Bowel Disease and in Controls. J. Clin. Microbiol. 38: 4373-4381 [Abstract] [Full Text]  
  • Whittington, R. J., Hope, A. F., Marshall, D. J., Taragel, C. A., Marsh, I. (2000). Molecular Epidemiology of Mycobacterium avium subsp. paratuberculosis: IS900 Restriction Fragment Length Polymorphism and IS1311 Polymorphism Analyses of Isolates from Animals and a Human in Australia. J. Clin. Microbiol. 38: 3240-3248 [Abstract] [Full Text]  
  • Coetsier, C., Vannuffel, P., Blondeel, N., Denef, J.-F., Cocito, C., Gala, J.-L. (2000). Duplex PCR for Differential Identification of Mycobacterium bovis, M. avium, and M. avium subsp. paratuberculosis in Formalin- Fixed Paraffin-Embedded Tissues from Cattle. J. Clin. Microbiol. 38: 3048-3054 [Abstract] [Full Text]  
  • Whittington, R. J., Fell, S., Walker, D., McAllister, S., Marsh, I., Sergeant, E., Taragel, C. A., Marshall, D. J., Links, I. J. (2000). Use of Pooled Fecal Culture for Sensitive and Economic Detection of Mycobacterium avium subsp. paratuberculosis Infection in Flocks of Sheep. J. Clin. Microbiol. 38: 2550-2556 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»