This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Limaye, A. P.
Right arrow Articles by Corey, L.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Limaye, A. P.
Right arrow Articles by Corey, L.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, April 1999, p. 1113-1116, Vol. 37, No. 4
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Detection of Epstein-Barr Virus DNA in Sera from Transplant Recipients with Lymphoproliferative Disorders

Ajit P. Limaye,1,* Meei-Li Huang,2 Ederlyn E. Atienza,2 James M. Ferrenberg,2 and Lawrence Corey2

Department of Laboratory Medicine & Medicine (Infectious Diseases), University of Washington,1 and Program in Infectious Diseases, Fred Hutchinson Cancer Research Center,2 Seattle, Washington 98195-7110

Received 9 October 1998/Returned for modification 7 December 1998/Accepted 13 January 1999

Early diagnosis of Epstein-Barr Virus (EBV)-associated posttransplant lymphoproliferative disease (PTLD) is important because many patients respond to reduction in immunosuppression, especially if PTLD is detected at an early stage. Previous studies have found elevated EBV DNA levels in blood from patients with PTLD, but these assays required isolation of cellular blood fractions and quantitation. We evaluated the presence of cell-free EBV DNA in serum from solid-organ transplant recipients as a marker for PTLD. Five of 6 transplant recipients with histopathologically documented PTLD had EBV DNA detected in serum at the time of diagnosis (sensitivity = 83%), compared with 0 of 16 matched transplant recipients without PTLD (specificity = 100%) (P < 0.001 [Fisher's exact test]). Furthermore, EBV DNA was detected in serum 8 and 52 months prior to the diagnosis of PTLD in two of three patients for whom stored sera were analyzed. Detection of EBV DNA in serum appears to be a useful marker for the early detection of PTLD in solid-organ transplant recipients. Further studies to define the role of such assays in evaluating solid-organ transplant patients at risk for PTLD are warranted.

* Corresponding author. Mailing address: University of Washington Medical Center, Dept. of Laboratory Medicine, Box 357110, 1959 N.E. Pacific St., Seattle, WA 98195-7110. Phone: (206) 598-2148. Fax: (206) 598-6189. E-mail: limaye{at}u.washington.edu.

Journal of Clinical Microbiology, April 1999, p. 1113-1116, Vol. 37, No. 4
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Wheless, S. A., Gulley, M. L., Raab-Traub, N., McNeillie, P., Neuringer, I. P., Ford, H. J., Aris, R. M. (2008). Post-transplantation Lymphoproliferative Disease: Epstein-Barr Virus DNA Levels, HLA-A3, and Survival. Am. J. Respir. Crit. Care Med. 178: 1060-1065 [Abstract] [Full Text]  
  • Hakim, H., Gibson, C., Pan, J., Srivastava, K., Gu, Z., Bankowski, M. J., Hayden, R. T. (2007). Comparison of Various Blood Compartments and Reporting Units for the Detection and Quantification of Epstein-Barr Virus in Peripheral Blood. J. Clin. Microbiol. 45: 2151-2155 [Abstract] [Full Text]  
  • Lai, K. K.-Y., Cook, L., Krantz, E. M., Corey, L., Jerome, K. R. (2005). Calibration Curves for Real-Time PCR. Clin. Chem. 51: 1132-1136 [Abstract] [Full Text]  
  • Schauer, E., Webber, S., Green, M., Rowe, D. (2004). Surface Immunoglobulin-Deficient Epstein-Barr Virus-Infected B Cells in the Peripheral Blood of Pediatric Solid-Organ Transplant Recipients. J. Clin. Microbiol. 42: 5802-5810 [Abstract] [Full Text]  
  • Wadowsky, R. M., Laus, S., Green, M., Webber, S. A., Rowe, D. (2003). Measurement of Epstein-Barr Virus DNA Loads in Whole Blood and Plasma by TaqMan PCR and in Peripheral Blood Lymphocytes by Competitive PCR. J. Clin. Microbiol. 41: 5245-5249 [Abstract] [Full Text]  
  • Andreone, P., Gramenzi, A., Lorenzini, S., Biselli, M., Cursaro, C., Pileri, S., Bernardi, M. (2003). Posttransplantation Lymphoproliferative Disorders. Arch Intern Med 163: 1997-2004 [Abstract] [Full Text]  
  • Jenkins, F. J., Rowe, D. T., Rinaldo, C. R. Jr. (2003). Herpesvirus Infections in Organ Transplant Recipients. CVI 10: 1-7 [Full Text]  
  • Rose, C., Green, M., Webber, S., Kingsley, L., Day, R., Watkins, S., Reyes, J., Rowe, D. (2002). Detection of Epstein-Barr Virus Genomes in Peripheral Blood B Cells from Solid-Organ Transplant Recipients by Fluorescence In Situ Hybridization. J. Clin. Microbiol. 40: 2533-2544 [Abstract] [Full Text]  
  • Lei, K. I. K., Chan, L. Y. S., Chan, W.-Y., Johnson, P. J., Lo, Y. M. D. (2002). Diagnostic and Prognostic Implications of Circulating Cell-free Epstein-Barr Virus DNA in Natural Killer/T-Cell Lymphoma. Clin. Cancer Res. 8: 29-34 [Abstract] [Full Text]  
  • Bharadwaj, M., Burrows, S. R., Burrows, J. M., Moss, D. J., Catalina, M., Khanna, R. (2001). Longitudinal dynamics of antigen-specific CD8+ cytotoxic T lymphocytes following primary Epstein-Barr virus infection. Blood 98: 2588-2589 [Full Text]  
  • van Esser, J. W. J., van der Holt, B., Meijer, E., Niesters, H. G. M., Trenschel, R., Thijsen, S. F. T., van Loon, A. M., Frassoni, F., Bacigalupo, A., Schaefer, U. W., Osterhaus, A. D. M. E., Gratama, J. W., Lowenberg, B., Verdonck, L. F., Cornelissen, J. J. (2001). Epstein-Barr virus (EBV) reactivation is a frequent event after allogeneic stem cell transplantation (SCT) and quantitatively predicts EBV-lymphoproliferative disease following T-cell-depleted SCT. Blood 98: 972-978 [Abstract] [Full Text]  
  • Stevens, S. J. C., Pronk, I., Middeldorp, J. M. (2001). Toward Standardization of Epstein-Barr Virus DNA Load Monitoring: Unfractionated Whole Blood as Preferred Clinical Specimen. J. Clin. Microbiol. 39: 1211-1216 [Abstract] [Full Text]  
  • Rose, C., Green, M., Webber, S., Ellis, D., Reyes, J., Rowe, D. (2001). Pediatric Solid-Organ Transplant Recipients Carry Chronic Loads of Epstein-Barr Virus Exclusively in the Immunoglobulin D-Negative B-Cell Compartment. J. Clin. Microbiol. 39: 1407-1415 [Abstract] [Full Text]  
  • Newstead, C. G. (2000). Lymphoproliferative disease post-renal transplantation. Nephrol Dial Transplant 15: 1913-1916 [Full Text]  
  • (2000). Management of herpes virus infections following transplantation. J Antimicrob Chemother 45: 729-748 [Full Text]  
  • Shotelersuk, K., Khorprasert, C., Sakdikul, S., Pornthanakasem, W., Voravud, N., Mutirangura, A. (2000). Epstein-Barr Virus DNA in Serum/Plasma as a Tumor Marker for Nasopharyngeal Cancer. Clin. Cancer Res. 6: 1046-1051 [Abstract] [Full Text]  
  • Niesters, H. G. M., van Esser, J., Fries, E., Wolthers, K. C., Cornelissen, J., Osterhaus, A. D. M. E. (2000). Development of a Real-Time Quantitative Assay for Detection of Epstein-Barr Virus. J. Clin. Microbiol. 38: 712-715 [Abstract] [Full Text]  
  • Beck, R., Westdörp, I., Jahn, G., Schäfer, H., Kanz, L., Einsele;, H., Limaye, A. P. (1999). Detection of Epstein-Barr Virus DNA in Plasma from Patients with Lymphoproliferative Disease after Allogeneic Bone Marrow or Peripheral Blood Stem Cell Transplantation. J. Clin. Microbiol. 37: 3430-3431 [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»