This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Kim, Y. B.
Right arrow Articles by Nishibuchi, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Kim, Y. B.
Right arrow Articles by Nishibuchi, M.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, April 1999, p. 1173-1177, Vol. 37, No. 4
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Identification of Vibrio parahaemolyticus Strains at the Species Level by PCR Targeted to the toxR Gene

Yung Bu Kim,1,dagger Jun Okuda,1 Chiho Matsumoto,1 Naoki Takahashi,2 Satoru Hashimoto,2 and Mitsuaki Nishibuchi1,*

Center for Southeast Asian Studies, Kyoto University, Yoshida, Sakyo-ku, Kyoto,1 and Kansai Airport Quarantine Station, Tajiri, Sennan, Osaka,2 Japan

Received 10 August 1998/Returned for modification 14 October 1998/Accepted 21 December 1998

The DNA colony hybridization test with the polynucleotide probe for Vibrio parahaemolyticus toxR gene was performed. All 373 strains of V. parahaemolyticus gave positive results, and the strains belonging to four other Vibrio species including Vibrio alginolyticus gave weakly positive results, suggesting that toxR sequence variation may reflect the phylogenetic relationships of Vibrio species. We then established a toxR-targeted PCR protocol for the specific detection of V. parahaemolyticus.

* Corresponding author. Mailing address: Center for Southeast Asian Studies, Kyoto University, 46 Shimoadachi-cho, Yoshida, Sakyo-ku, Kyoto 606-8501, Japan. Phone: 81-75-753-7367. Fax: 81-75-753-7350. E-mail: nishibuc{at}med.kyoto-u.ac.jp.

dagger Present address: Department of Microbiology, College of Medicine, Pusan National University, Pusan 602-739, Korea.

Journal of Clinical Microbiology, April 1999, p. 1173-1177, Vol. 37, No. 4
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Alam, M., Chowdhury, W. B., Bhuiyan, N. A., Islam, A., Hasan, N. A., Nair, G. B., Watanabe, H., Siddique, A. K., Huq, A., Sack, R. B., Akhter, M. Z., Grim, C. J., Kam, K.-M., Luey, C. K. Y., Endtz, H. P., Cravioto, A., Colwell, R. R. (2009). Serogroup, Virulence, and Genetic Traits of Vibrio parahaemolyticus in the Estuarine Ecosystem of Bangladesh. Appl. Environ. Microbiol. 75: 6268-6274 [Abstract] [Full Text]  
  • Ottaviani, D., Leoni, F., Rocchegiani, E., Santarelli, S., Canonico, C., Masini, L., DiTrani, V., Carraturo, A. (2008). First Clinical Report of Pandemic Vibrio parahaemolyticus O3:K6 Infection in Italy. J. Clin. Microbiol. 46: 2144-2145 [Full Text]  
  • Wootipoom, N., Bhoopong, P., Pomwised, R., Nishibuchi, M., Ishibashi, M., Vuddhakul, V. (2007). A decrease in the proportion of infections by pandemic Vibrio parahaemolyticus in Hat Yai Hospital, southern Thailand. J Med Microbiol 56: 1630-1638 [Abstract] [Full Text]  
  • Bhoopong, P., Palittapongarnpim, P., Pomwised, R., Kiatkittipong, A., Kamruzzaman, M., Nakaguchi, Y., Nishibuchi, M., Ishibashi, M., Vuddhakul, V. (2007). Variability of Properties of Vibrio parahaemolyticus Strains Isolated from Individual Patients. J. Clin. Microbiol. 45: 1544-1550 [Abstract] [Full Text]  
  • Serichantalergs, O., Bhuiyan, N. A., Nair, G. B., Chivaratanond, O., Srijan, A., Bodhidatta, L., Anuras, S., Mason, C. J. (2007). The dominance of pandemic serovars of Vibrio parahaemolyticus in expatriates and sporadic cases of diarrhoea in Thailand, and a new emergent serovar (O3 : K46) with pandemic traits. J Med Microbiol 56: 608-613 [Abstract] [Full Text]  
  • Tracz, D. M., Backhouse, P. G., Olson, A. B., McCrea, J. K., Walsh, J. A., Ng, L.-K., Gilmour, M. W. (2007). Rapid detection of Vibrio species using liquid microsphere arrays and real-time PCR targeting the ftsZ locus. J Med Microbiol 56: 56-65 [Abstract] [Full Text]  
  • Hayashi, S., Okura, M., Osawa, R. (2006). Soft-Agar-Coated Filter Method for Early Detection of Viable and Thermostable Direct Hemolysin (TDH)- or TDH-Related Hemolysin-Producing Vibrio parahaemolyticus in Seafood.. Appl. Environ. Microbiol. 72: 4576-4582 [Abstract] [Full Text]  
  • Chakraborty, R., Sinha, S., Mukhopadhyay, A. K., Asakura, M., Yamasaki, S., Bhattacharya, S. K., Nair, G. B., Ramamurthy, T. (2006). Species-specific identification of Vibrio fluvialis by PCR targeted to the conserved transcriptional activation and variable membrane tether regions of the toxR gene. J Med Microbiol 55: 805-808 [Full Text]  
  • Chakraborty, R., Chakraborty, S., De, K., Sinha, S., Mukhopadhyay, A. K, Khanam, J., Ramamurthy, T., Takeda, Y., Bhattacharya, S. K, Nair, G B. (2005). Cytotoxic and cell vacuolating activity of Vibrio fluvialis isolated from paediatric patients with diarrhoea. J Med Microbiol 54: 707-716 [Abstract] [Full Text]  
  • Deepanjali, A., Kumar, H. S., Karunasagar, I., Karunasagar, I. (2005). Seasonal Variation in Abundance of Total and Pathogenic Vibrio parahaemolyticus Bacteria in Oysters along the Southwest Coast of India. Appl. Environ. Microbiol. 71: 3575-3580 [Abstract] [Full Text]  
  • Ansaruzzaman, M., Lucas, M., Deen, J. L., Bhuiyan, N. A., Wang, X.-Y., Safa, A., Sultana, M., Chowdhury, A., Nair, G. B., Sack, D. A., von Seidlein, L., Puri, M. K., Ali, M., Chaignat, C.-L., Clemens, J. D., Barreto, A. (2005). Pandemic Serovars (O3:K6 and O4:K68) of Vibrio parahaemolyticus Associated with Diarrhea in Mozambique: Spread of the Pandemic into the African Continent. J. Clin. Microbiol. 43: 2559-2562 [Abstract] [Full Text]  
  • Ramos, J. L., Martinez-Bueno, M., Molina-Henares, A. J., Teran, W., Watanabe, K., Zhang, X., Gallegos, M. T., Brennan, R., Tobes, R. (2005). The TetR Family of Transcriptional Repressors. Microbiol. Mol. Biol. Rev. 69: 326-356 [Abstract] [Full Text]  
  • Cabrera-Garcia, M. E., Vazquez-Salinas, C., Quinones-Ramirez, E. I. (2004). Serologic and Molecular Characterization of Vibrio parahaemolyticus Strains Isolated from Seawater and Fish Products of the Gulf of Mexico. Appl. Environ. Microbiol. 70: 6401-6406 [Abstract] [Full Text]  
  • Martinez-Urtaza, J., Lozano-Leon, A., DePaola, A., Ishibashi, M., Shimada, K., Nishibuchi, M., Liebana, E. (2004). Characterization of Pathogenic Vibrio parahaemolyticus Isolates from Clinical Sources in Spain and Comparison with Asian and North American Pandemic Isolates. J. Clin. Microbiol. 42: 4672-4678 [Abstract] [Full Text]  
  • Williams, T. L., Musser, S. M., Nordstrom, J. L., DePaola, A., Monday, S. R. (2004). Identification of a Protein Biomarker Unique to the Pandemic O3:K6 Clone of Vibrio parahaemolyticus. J. Clin. Microbiol. 42: 1657-1665 [Abstract] [Full Text]  
  • Chowdhury, N. R., Stine, O. C., Morris, J. G., Nair, G. B. (2004). Assessment of Evolution of Pandemic Vibrio parahaemolyticus by Multilocus Sequence Typing. J. Clin. Microbiol. 42: 1280-1282 [Abstract] [Full Text]  
  • Hara-Kudo, Y., Sugiyama, K., Nishibuchi, M., Chowdhury, A., Yatsuyanagi, J., Ohtomo, Y., Saito, A., Nagano, H., Nishina, T., Nakagawa, H., Konuma, H., Miyahara, M., Kumagai, S. (2003). Prevalence of Pandemic Thermostable Direct Hemolysin-Producing Vibrio parahaemolyticus O3:K6 in Seafood and the Coastal Environment in Japan. Appl. Environ. Microbiol. 69: 3883-3891 [Abstract] [Full Text]  
  • Singh, D. V., Isac, S. R., Colwell, R. R. (2002). Development of a Hexaplex PCR Assay for Rapid Detection of Virulence and Regulatory Genes in Vibrio cholerae and Vibrio mimicus. J. Clin. Microbiol. 40: 4321-4324 [Abstract] [Full Text]  
  • Bhuiyan, N. A., Ansaruzzaman, M., Kamruzzaman, M., Alam, K., Chowdhury, N. R., Nishibuchi, M., Faruque, S. M., Sack, D. A., Takeda, Y., Nair, G. B. (2002). Prevalence of the Pandemic Genotype of Vibrio parahaemolyticus in Dhaka, Bangladesh, and Significance of Its Distribution across Different Serotypes. J. Clin. Microbiol. 40: 284-286 [Abstract] [Full Text]  
  • Hara-Kudo, Y., Nishina, T., Nakagawa, H., Konuma, H., Hasegawa, J., Kumagai, S. (2001). Improved Method for Detection of Vibrio parahaemolyticus in Seafood. Appl. Environ. Microbiol. 67: 5819-5823 [Abstract] [Full Text]  
  • Okuda, J., Nakai, T., Chang, P. S., Oh, T., Nishino, T., Koitabashi, T., Nishibuchi, M. (2001). The toxR Gene of Vibrio (Listonella) anguillarum Controls Expression of the Major Outer Membrane Proteins but Not Virulence in a Natural Host Model. Infect. Immun. 69: 6091-6101 [Abstract] [Full Text]  
  • Provenzano, D., Lauriano, C. M., Klose, K. E. (2001). Characterization of the Role of the ToxR-Modulated Outer Membrane Porins OmpU and OmpT in Vibrio cholerae Virulence. J. Bacteriol. 183: 3652-3662 [Abstract] [Full Text]  
  • Nandi, B., Nandy, R. K., Mukhopadhyay, S., Nair, G. B., Shimada, T., Ghose, A. C. (2000). Rapid Method for Species-Specific Identification of Vibrio cholerae Using Primers Targeted to the Gene of Outer Membrane Protein OmpW. J. Clin. Microbiol. 38: 4145-4151 [Abstract] [Full Text]  
  • Vuddhakul, V., Nakai, T., Matsumoto, C., Oh, T., Nishino, T., Chen, C.-H., Nishibuchi, M., Okuda, J. (2000). Analysis of gyrB and toxR Gene Sequences of Vibrio hollisae and Development of gyrB- and toxR-Targeted PCR Methods for Isolation of V. hollisae from the Environment and Its Identification. Appl. Environ. Microbiol. 66: 3506-3514 [Abstract] [Full Text]  
  • Vuddhakul, V., Chowdhury, A., Laohaprertthisan, V., Pungrasamee, P., Patararungrong, N., Thianmontri, P., Ishibashi, M., Matsumoto, C., Nishibuchi, M. (2000). Isolation of a Pandemic O3:K6 Clone of a Vibrio parahaemolyticus Strain from Environmental and Clinical Sources in Thailand. Appl. Environ. Microbiol. 66: 2685-2689 [Abstract] [Full Text]  
  • Matsumoto, C., Okuda, J., Ishibashi, M., Iwanaga, M., Garg, P., Rammamurthy, T., Wong, H.-C., Depaola, A., Kim, Y. B., Albert, M. J., Nishibuchi, M. (2000). Pandemic Spread of an O3:K6 Clone of Vibrio parahaemolyticus and Emergence of Related Strains Evidenced by Arbitrarily Primed PCR and toxRS Sequence Analyses. J. Clin. Microbiol. 38: 578-585 [Abstract] [Full Text]  
  • Osorio, C. R., Klose, K. E. (2000). A Region of the Transmembrane Regulatory Protein ToxR That Tethers the Transcriptional Activation Domain to the Cytoplasmic Membrane Displays Wide Divergence among Vibrio Species. J. Bacteriol. 182: 526-528 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»